Chromosomics: detection of numerical and structural alterations in all 24 human chromosomes simultaneously using a novel OctoChrome FISH assay.

Journal of Visualized Experiments : JoVE
Zhiying Ji, Luoping Zhang

Abstract

Fluorescence in situ hybridization (FISH) is a technique that allows specific DNA sequences to be detected on metaphase or interphase chromosomes in cell nuclei(1). The technique uses DNA probes with unique sequences that hybridize to whole chromosomes or specific chromosomal regions, and serves as a powerful adjunct to classic cytogenetics. For instance, many earlier studies reported the frequent detection of increased chromosome aberrations in leukemia patients related with benzene exposure, benzene-poisoning patients, and healthy workers exposed to benzene, using classic cytogenetic analysis(2). Using FISH, leukemia-specific chromosomal alterations have been observed to be elevated in apparently healthy workers exposed to benzene(3-6), indicating the critical roles of cytogentic changes in benzene-induced leukemogenesis. Generally, a single FISH assay examines only one or a few whole chromosomes or specific loci per slide, so multiple hybridizations need to be conducted on multiple slides to cover all of the human chromosomes. Spectral karyotyping (SKY) allows visualization of the whole genome simultaneously, but the requirement for special software and equipment limits its application(7). Here, we describe a novel FISH ...Continue Reading

References

Mar 22, 2001·Clinical Genetics·J Bayani, J A Squire
Feb 16, 2002·Critical Reviews in Toxicology·Luoping ZhangMartyn T Smith
Jun 17, 2003·Journal of Cell Science·Jeffrey M Levsky, Robert H Singer
Jun 23, 2007·Environmental and Molecular Mutagenesis·Luoping ZhangMartyn T Smith
Jan 15, 2010·Annual Review of Public Health·Martyn T Smith

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Citations

May 16, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Kumar Selvarajoo
Feb 28, 2014·Annals of the New York Academy of Sciences·Cliona M McHaleLuoping Zhang
Feb 17, 2019·International Journal of Molecular Sciences·Thomas Liehr
Mar 11, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Martyn T SmithMark Fielden

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