Chronic alcohol exposure affects pancreatic acinar mitochondrial thiamin pyrophosphate uptake: studies with mouse 266-6 cell line and primary cells

American Journal of Physiology. Gastrointestinal and Liver Physiology
Padmanabhan SrinivasanHamid M Said

Abstract

Thiamin is essential for normal metabolic activity of all mammalian cells, including those of the pancreas. Cells obtain thiamin from their surroundings and enzymatically convert it into thiamin pyrophosphate (TPP) in the cytoplasm; TPP is then taken up by mitochondria via a specific carrier the mitochondrial TPP transporter (MTPPT; product of the SLC25A19 gene). Chronic alcohol exposure negatively impacts the health of pancreatic acinar cells (PAC), but its effect on physiological/molecular parameters of MTPPT is not known. We addressed this issue using mouse pancreatic acinar tumor cell line 266-6 and primary PAC of wild-type and transgenic mice carrying the SLC25A19 promoter that were fed alcohol chronically. Chronic alcohol exposure of 266-6 cells (but not to its nonoxidative metabolites ethyl palmitate and ethyl oleate) led to a significant inhibition in mitochondrial TPP uptake, which was associated with a decreased expression of MTPPT protein, mRNA, and activity of the SLC25A19 promoter. Similarly, chronic alcohol feeding of mice led to a significant inhibition in expression of MTPPT protein, mRNA, heterogeneous nuclear RNA, as well as in activity of SLC25A19 promoter in PAC. While chronic alcohol exposure did not affect...Continue Reading

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Citations

Aug 16, 2016·Gastroenterology·Rupjyoti TalukdarAshok K Saluja
Apr 29, 2016·American Journal of Physiology. Cell Physiology·Veedamali S SubramanianHamid M Said
Mar 22, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Padmanabhan SrinivasanHamid M Said
Sep 22, 2017·American Journal of Physiology. Cell Physiology·Kasin Yadunandam AnandamHamid M Said
Jul 21, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kalidas RamamoorthyHamid M Said
Jul 22, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Subrata SabuiHamid M Said
Sep 5, 2019·American Journal of Physiology. Cell Physiology·Padmanabhan SrinivasanHamid M Said
Aug 26, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lindsey KennedyGianfranco Alpini
Aug 7, 2021·Biochemical and Biophysical Research Communications·Qinhao ShenGuanghuai Yao

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