Chronic Alcohol Exposure and the Circadian Clock Mutation Exert Tissue-Specific Effects on Gene Expression in Mouse Hippocampus, Liver, and Proximal Colon

Alcoholism, Clinical and Experimental Research
Keith C SummaFred W Turek

Abstract

Chronic alcohol exposure exerts numerous adverse effects, although the specific mechanisms underlying these negative effects on different tissues are not completely understood. Alcohol also affects core properties of the circadian clock system, and it has been shown that disruption of circadian rhythms confers vulnerability to alcohol-induced pathology of the gastrointestinal barrier and liver. Despite these findings, little is known of the molecular interactions between alcohol and the circadian clock system, especially regarding implications for tissue-specific susceptibility to alcohol pathologies. The aim of this study was to identify changes in expression of genes relevant to alcohol pathologies and circadian clock function in different tissues in response to chronic alcohol intake. Wild-type and circadian Clock(Δ19) mutant mice were subjected to a 10-week chronic alcohol protocol, after which hippocampal, liver, and proximal colon tissues were harvested for gene expression analysis using a custom-designed multiplex magnetic bead hybridization assay that provided quantitative assessment of 80 mRNA targets of interest, including 5 housekeeping genes and a predetermined set of 75 genes relevant for alcohol pathology and circ...Continue Reading

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Citations

Sep 30, 2017·Alcoholism, Clinical and Experimental Research·Booker T DavisAli Keshavarzian
Jun 1, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shannon M Bailey
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Jan 15, 2021·Pharmacology, Biochemistry, and Behavior·Eduardo K TamuraNatalí N Guerrero-Vargas

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