Abstract
Chronic bile duct ligation (BDL) is a useful model of cirrhosis. However, its parallel plasma and liver changes in levels of cytokines and nitric oxide (NO), involved in liver damage, remain unknown. The aims of this work were to quantify both the plasma and hepatic levels of five cytokines and NO in cirrhotic rats, 28 days after bile BDL, and to analyze their relationship with liver damage markers. One group of male Wistar rats was bile duct ligated and another group was sham operated, both groups were sacrificed 28 days after BDL. Plasma and liver cytokines, tumor necrosis factor-alpha (TNF-alpha), interleukin-6, -1beta, -10 (IL-6, -1beta, -10) and interferon-gamma (IFN-gamma), were measured by ELISA. Plasma and hepatic NO was determined as NO(2)(-)+NO(3)(-) by an enzymatic method. Alkaline phosphatase, gamma-glutamyl transpeptidase, alanine aminotransferase and bilirubins were determined in plasma. Collagen, lipid peroxidation and glycogen were quantified in liver. Two histopathological staining techniques were performed. BDL-induced cirrhosis was corroborated by the elevated liver damage markers and histopathological analysis. Chronic BDL significantly increased (P<0.05) most of plasma and hepatic cytokine levels and dimini...Continue Reading
References
Apr 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F BulleG Guellaën
Jan 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M E De BroeR J Wieme
Nov 1, 1966·Clinica Chimica Acta; International Journal of Clinical Chemistry·A Szewczuk
Nov 1, 1994·Journal of Applied Toxicology : JAT·P Muriel, O R Suarez
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C GuarnerS Moncada
Jul 15, 1996·The Biochemical Journal·E D CeppiM A Titheradge
May 1, 1996·Journal of Hepatology·L LlorentJ Alcocer-Varela
Oct 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J NapoliG W McCaughan
Feb 7, 1998·The Journal of Biological Chemistry·S C WangH Tsukamoto
Feb 7, 1998·Journal of Hepatology·K J SimpsonS L Kunkel
Apr 18, 1998·The Biochemical Journal·F SprangersA J Meijer
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A SchroederP C Kuo
Sep 29, 1998·Biochemical Pharmacology·P Muriel
Oct 17, 1998·Toxicology Letters·L A DeCiccoK R Hornbrook
Apr 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R MarleyK Moore
May 4, 1999·Clinical and Experimental Pharmacology & Physiology·M PlebaniD Infantolino
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M G Clemens
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A A NanjiG L Su
Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D HarryK Moore
Nov 26, 1999·Biochimica Et Biophysica Acta·V Barón, P Muriel
Mar 29, 2000·Gastroenterology·D R NelsonG L Davis
May 8, 2000·Journal of Applied Toxicology : JAT·P Muriel
Jun 26, 2001·Liver·R Farzaneh-Far, K Moore
Nov 8, 2002·Biochimica Et Biophysica Acta·Eithan Galun, Jonathan H Axelrod
Mar 26, 2003·Journal of Gastroenterology and Hepatology·Orhan TarçinAhmet K Gürbüz
Aug 5, 2003·Liver International : Official Journal of the International Association for the Study of the Liver·Pablo Muriel, Rogelio Deheza
Nov 1, 1960·Analytical Biochemistry·D J PROCKOP, S UDENFRIEND
Oct 7, 2003·Current Molecular Medicine·Tracy ChenTimothy R Billiar
Citations
Jan 12, 2007·Pediatric Surgery International·Göksel SenerNursal Gedik
Nov 19, 2008·Fibrogenesis & Tissue Repair·María-Angeles AllerJaime Arias
Apr 22, 2014·International Immunopharmacology·Eduardo Fernández-MartínezMineko Shibayama
Oct 4, 2011·Journal of the Chinese Medical Association : JCMA·Hsian-Guey HsiehShou-Dong Lee
Feb 23, 2010·Brain Research·Konstantinos FaropoulosDimitris Konstantinou
Dec 25, 2009·The Journal of Surgical Research·José García-DominguezJaime Arias
Dec 20, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Eduardo Fernández-MartínezVíctor Tsutsumi
May 13, 2008·Gastroenterology·Arun J SanyalVincente Arroyo
Dec 19, 2006·Archives of Medical Research·Semra Doğru-AbbasoğluMüjdat Uysal
Sep 14, 2015·Neuroscience and Biobehavioral Reviews·David Chun-Hei PoonRaymond Chuen-Chung Chang
Oct 18, 2007·The Journal of Pharmacy and Pharmacology·Hale Z TokluGoksel Sener
Jul 16, 2008·Journal of Applied Toxicology : JAT·Adriana Rodríguez-RiveraPablo Muriel
Oct 22, 2008·Journal of Applied Toxicology : JAT·Pablo Muriel
Jan 4, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Ajay C DonepudiAngela L Slitt
Jan 1, 2008·European Journal of Pharmacology·Taha GholipourAhmad Reza Dehpour
Sep 30, 2015·PloS One·Yusuke KawazoeTakashi Takata
Aug 20, 2010·Arquivos De Gastroenterologia·Ana Regina L RamosThemis Reverbel da Silveira
Nov 26, 2009·Chemistry & Biodiversity·Gregory J AngerMicheline Piquette-Miller
Apr 2, 2010·Nanotechnology·Danying ZhangYuanfang Liu
Jul 13, 2019·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Kyoung Wan KwonUy Dong Sohn
Sep 7, 2019·Journal of Food Biochemistry·Eduardo Fernández-MartínezNury Pérez-Hernández
Feb 23, 2019·PloS One·Gisel S MiszczukFernando A Crocenzi
Feb 23, 2011·Human & Experimental Toxicology·Ali ZandiehAhmad Reza Dehpour
Dec 9, 2016·Pharmaceutical Biology·Javier Morales-LópezEduardo Fernández-Martínez
Sep 25, 2019·International Journal of Molecular Sciences·Luis E Soria-JassoEduardo Fernández-Martínez
Mar 3, 2021·Life Sciences·Zeinab EsmaeiliFarahnaz Jazaeri
Jun 27, 2021·Journal of Food Biochemistry·Armagan Begum OzelAysen Yarat