Chronic Electrical Stimulation Promotes the Excitability and Plasticity of ESC-derived Neurons following Glutamate-induced Inhibition In vitro

Scientific Reports
Charles-Francois V LatchoumaneLohitash Karumbaiah

Abstract

Functional electrical stimulation (FES) is rapidly gaining traction as a therapeutic tool for mediating the repair and recovery of the injured central nervous system (CNS). However, the underlying mechanisms and impact of these stimulation paradigms at a molecular, cellular and network level remain largely unknown. In this study, we used embryonic stem cell (ESC)-derived neuron and glial co-cultures to investigate network maturation following acute administration of L-glutamate, which is a known mediator of excitotoxicity following CNS injury. We then modulated network maturation using chronic low frequency stimulation (LFS) and direct current stimulation (DCS) protocols. We demonstrated that L-glutamate impaired the rate of maturation of ESC-derived neurons and glia immediately and over a week following acute treatment. The administration of chronic LFS and DCS protocols individually following L-glutamate infusion significantly promoted the excitability of neurons as well as network synchrony, while the combination of LFS/DCS did not. qRT-PCR analysis revealed that LFS and DCS alone significantly up-regulated the expression of excitability and plasticity-related transcripts encoding N-methyl-D-aspartate (NMDA) receptor subunit...Continue Reading

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Citations

Aug 15, 2018·International Journal of Molecular Sciences·Ekaterina V MedvedevaAndrei S Chagin
Nov 5, 2019·Frontiers in Neuroscience·Jose L Perez VelazquezRamon Guevara Erra
Jan 30, 2021·Frontiers in Neuroscience·Austin P Passaro, Steven L Stice
Apr 10, 2021·APL Bioengineering·Danielle WarrenJeremy M Crook
Dec 9, 2019·ACS Biomaterials Science & Engineering·Rongrong GuoMingliang Tang

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BETA
PCR

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Volocity
NoStim
MATLAB scripts
MEA
SigmaPlot
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