Chronic endothelin-1 infusion causes adipocyte hyperplasia in rats

Obesity
Chih-Chan LienChi-Chang Juan

Abstract

The aim of this study was to investigate the regulatory mechanism of endothelin-1 (ET-1), an endothelium-derived vasoconstrictor, on adipogenesis in vitro and in vivo. 3T3-L1 preadipocytes were used to explore the mechanisms mediating ET-1 actions on preadipocyte proliferation and adipocyte differentiation. To investigate the in vivo effect of ET-1, male Sprague-Dawley rats were infused with ET-1 or saline for 4 weeks via intraperitoneally implanted osmotic pumps, and the fat pad weight and adipocyte size of adipose tissues were measured. ET-1 stimulated preadipocyte proliferation and increased the cell number at the mitotic clonal expansion stage of adipocyte differentiation via the endothelin A receptor (ETAR) and activation of the protein kinase C (PKC) pathway. ET-1, via ETAR, inhibited adipocyte differentiation partially through an ERK-dependent pathway. Furthermore, no significant difference in the body weight and fat pad weight was observed in either ET-1- or saline-infused rats. Compared with saline-infused rats, the adipocyte cell number was significantly increased but the adipocyte size was significantly decreased in ET-1-infused rats. Chronic ET-1 infusion increased the number of small adipocytes without the change o...Continue Reading

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Citations

Jul 5, 2016·International Journal of Molecular Sciences·Francisco Javier Ruiz-OjedaConcepción María Aguilera
Jul 7, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Haley N JenkinsJoshua S Speed
Apr 29, 2016·Oncology Letters·Shaozhong WangHuiling Zhong

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