Chronic ethanol exposure of human pancreatic normal ductal epithelial cells induces cancer stem cell phenotype through SATB2.

Journal of Cellular and Molecular Medicine
Wei YuRakesh K Srivastava

Abstract

The incidence of pancreatic cancer is on the rise. Risk factors for pancreatic cancer include alcohol toxicity and metabolic conditions such as obesity, hypertension, dyslipidaemia, insulin resistance and type 2 diabetes. However, the molecular mechanism by which chronic alcohol consumption contributes to pancreatic cancer is not well understood. The purpose of the study was to demonstrate the effects of long-term chronic ethanol exposure on the transformation of human pancreatic normal ductal epithelial (HPNE) cells. Our data showed that ethanol-transformed HPNE cells were more progressively transformed exhibiting spheroids and colonies, and anchorage-independent growth. These transformed cells contained high levels of reactive oxygen species and induced SATB2 expression. Furthermore, during ethanol-induced cellular transformation, cells gained the phenotypes of cancer stem cells (CSCs) by expressing pluripotency maintaining factors (Oct4, Sox2, cMyc and KLF4) and stem cell markers (CD24, CD44 and CD133). Ethanol-induced SATB2 can bind to the promoters of KLF4, Oct4, cMyc, Sox2, Bcl-2 and XIAP genes. Suppression of SATB2 expression in ethanol-transformed HPNE cells inhibited cell proliferation, colony formation and markers of ...Continue Reading

References

Feb 13, 1992·The New England Journal of Medicine·A L Warshaw, C Fernández-del Castillo
Jan 1, 1984·The Medical Clinics of North America·J H Breeden
Mar 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·M P Longnecker, S M Enger
Nov 4, 1998·Alcoholism, Clinical and Experimental Research·U Ringborg
Jun 25, 2002·Best Practice & Research. Clinical Gastroenterology·Conor J MageeJohn P Neoptolemos
Jul 24, 2002·Gastroenterology Clinics of North America·David C Whitcomb, Katherine Pogue-Geile
Dec 3, 2002·Nature Reviews. Cancer·Nabeel Bardeesy, Ronald A DePinho
Apr 15, 2003·Nature Genetics·Shutao CaiTerumi Kohwi-Shigematsu
Aug 12, 2003·International Journal of Gastrointestinal Cancer·Donghui Li, Li Jiao
Apr 9, 2004·The Proceedings of the Nutrition Society·Gudrun PöschlHelmut K Seitz
Apr 15, 2004·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·G Pöschl, H K Seitz
Aug 2, 2005·Alcohol·Vay Liang W GoStephen J Pandol
Jul 25, 2006·Oral Oncology·Johanna KurkivuoriMikko Salaspuro
Jun 16, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Sharmila ShankarRakesh K Srivastava
Mar 13, 2008·The European Journal of Neuroscience·Andrea B GyorgyDenes V Agoston
Oct 7, 2009·Nature Reviews. Gastroenterology & Hepatology·Sara RaimondiAlbert B Lowenfels
Apr 8, 2010·Cancer Causes & Control : CCC·Dominique S MichaudRachael Z Stolzenberg-Solomon
Aug 10, 2010·Current Opinion in Gastroenterology·Matthew J DiMagno, Eugene P DiMagno
Jul 8, 2011·Digestive Diseases·Claudia NitscheMarkus M Lerch
Jul 29, 2011·International Journal of Cancer. Journal International Du Cancer·Su-Ni TangRakesh K Srivastava
Nov 30, 2011·The Journal of Biological Chemistry·Kazuo AsanomaM A Karim Rumi
Sep 4, 2012·Journal of Hepatology·Tung-Ming Leung, Natalia Nieto
Feb 23, 2013·World Journal of Gastroenterology : WJG·Marta Herreros-VillanuevaLuis Bujanda
Apr 30, 2013·Gastroenterology·Dhiraj Yadav, Albert B Lowenfels
May 25, 2013·Histopathology·James R Conner, Jason L Hornick
Jul 3, 2013·Nutrition Reviews·Elhaseen E ElaminDaisy M Jonkers
Aug 15, 2013·International Journal of Molecular Sciences·Yolande AsaraRoberto Madeddu
May 16, 2015·Biochimica Et Biophysica Acta·Divya SinghSharmila Shankar
Feb 9, 2017·Stem Cell Research·Wei YuRakesh K Srivastava
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

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Citations

Sep 6, 2019·Molecular Biology Reports·Arkadiusz GzilŁukasz Szylberg
Sep 5, 2020·Journal of Cellular and Molecular Medicine·Sanjit K RoyRakesh K Srivastava
Mar 2, 2021·World Journal of Gastrointestinal Oncology·Yang ZhouHong-Wu Xin
Jul 25, 2021·Cancers·Francisco D Rodriguez, Rafael Coveñas

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Methods Mentioned

BETA
immunoprecipitation
transfection
electrophoresis

Software Mentioned

PRISM

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