Chronic exercise increases both inducible and endothelial nitric oxide synthase gene expression in endothelial cells of rat aorta

Journal of Biomedical Science
Ai-Lun YangHsiun-Ing Chen

Abstract

Chronic exercise upregulates endothelial nitric oxide synthase (eNOS) gene expression. Whether the expression of inducible nitric oxide synthase (iNOS) is affected by exercise is unknown. We therefore investigated the effects of chronic exercise on iNOS and eNOS expression in isolated rat aortic endothelial and smooth muscle cells separately. Five-week-old male Wistar rats were randomly divided into control and exercise groups. After 10 weeks of running training, animals were sacrificed under ether anesthesia. The standard curve quantitative competitive reverse transcriptase-polymerase chain reaction method was used to quantify NOS mRNA expression in isolated endothelial/smooth muscle cells. To evaluate the functional role of iNOS, we examined phenylephrine-induced vascular responses with or without pretreatment with aminoguanidine. We found that (1) expression of iNOS and eNOS mRNA in endothelial cells was increased by chronic exercise and (2) chronic exercise blunted phenylephrine-induced vascular responses, probably by increasing NO release via iNOS. Our results show that chronic exercise increases both iNOS and eNOS gene expression in endothelium. These alterations may be partially responsible for the change in vascular res...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M W RadomskiS Moncada
Dec 1, 1991·Journal of Applied Physiology·C M TiptonS B Williams
Apr 1, 1989·Journal of Applied Physiology·J C RussellD P Boer
Mar 1, 1985·Progress in Cardiovascular Diseases·T F Schaible, J Scheuer
Nov 1, 1993·British Journal of Pharmacology·M J GriffithsT W Evans
Apr 29, 1994·Biochemical and Biophysical Research Communications·Y NunokawaS Tanaka
Feb 26, 1993·Biochemical and Biophysical Research Communications·Y NunokawaS Tanaka
Sep 1, 1993·Journal of Applied Physiology·M D DelpM H Laughlin
Jan 1, 1993·Acta Physiologica Scandinavica·H I ChenW C Chang
Jun 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H I Chen, H T Li
Dec 31, 1997·Medicine and Science in Sports and Exercise·M D Delp, M H Laughlin
Aug 4, 1998·The American Journal of Physiology·C L CannonK Strange
Feb 23, 1999·Circulation·R J Shephard, G J Balady
Jul 27, 2000·Medicine and Science in Sports and Exercise·A M NiessE Fehrenbach
Nov 18, 2000·American Journal of Physiology. Cell Physiology·W GosgnachJ B Michel

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Citations

Oct 22, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Reid HaywardKim C. Westerlind
Jun 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Claudio NapoliLouis J Ignarro
Mar 17, 2006·Medicine and Science in Sports and Exercise·Jeffrey L Jasperse, M Harold Laughlin
Sep 24, 2005·Fundamental & Clinical Pharmacology·Roger De MoraesEduardo Tibiriçá
Nov 1, 2008·Journal of Periodontal Research·Y-T ChinE Fu
Jan 5, 2011·Biochemical and Biophysical Research Communications·Shu-Hui SuHsiun-ing Chen
Nov 23, 2017·Frontiers in Cardiovascular Medicine·Saradhadevi VaradharajJay L Zweier
Aug 20, 2014·Metabolic Syndrome and Related Disorders·Marcus Vinicius MachadoEduardo Tibiriçá
Mar 10, 2017·Anesthesia and Analgesia·Robert H Thiele
Apr 20, 2004·Journal of Applied Physiology·Roger De MoraesEduardo Tibiriçá
Aug 17, 2005·Hypertension·Tatiana Sousa CunhaFernanda Klein Marcondes
Jun 14, 2006·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Nicolas TordiPascal Laurant
Mar 6, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Michał K ZarobkiewiczBarbara Jodłowska-Jędrych
Mar 30, 2011·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T Bruder-Nascimento, S Cordellini
Jul 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Chauying J JenHsiun-ing Chen
Jun 22, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Justin D La FavorRobert C Hickner
Jul 17, 2003·European Journal of Clinical Investigation·Z RadákP Ferdinandy
Aug 7, 2021·Biomolecules·Magdalena WiecekJadwiga Szymura
Aug 12, 2008·Vascular Pharmacology·Anuran ChatterjeeJohn D Catravas

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