Chronic fluoxetine rescues changes in plasma membrane density of 5-HT1A autoreceptors and serotonin transporters in the olfactory bulbectomy rodent model of depression

Neuroscience
Mustapha RiadJean-Claude Lacaille

Abstract

Reduced serotonin (5-HT) neurotransmission is postulated to underlie the pathogenesis of depression. The serotonin transporter (SERT) and 5-HT1A auto-receptors act in concert to ensure homeostasis of serotonin (5-HT) neurotransmission and regulation of their cell surface expression represent efficient mechanisms to maintain this homeostasis. Thus, we investigated the changes in the subcellular distribution of SERT and 5-HT1A receptors (5-HT1AR) in the rat olfactory bulbectomy model of depression using immuno-gold labeling and electron microscopy, and examined the effect of chronic treatment with the antidepressant, fluoxetine, a serotonin reuptake inhibitor, on the subcellular distribution of SERT and 5-HT1AR. The density of plasma membrane labeling of 5-HT1A auto-receptors on dendrites of dorsal raphe neurons was increased after bulbectomy, but the 5-HT1A hetero-receptor membrane labeling on dendrites of CA3 hippocampal neurons was not. The density of membrane labeling of SERTs was increased both in dendrites of dorsal raphe neuron and axon terminals in the hippocampus after bulbectomy. However, the proportion of 5-HT1AR and SERT membrane labeling relative to total labeling was unchanged, suggesting an increase in protein leve...Continue Reading

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Citations

May 14, 2020·Journal of Neurophysiology·Tyler R SizemoreAndrew M Dacks
Oct 5, 2019·Journal of Neural Transmission·Barbara PlanchezCatherine Belzung
May 1, 2020·Psychiatry Research·Brent M Kious, Amanda V Bakian
Dec 11, 2021·The International Journal of Neuropsychopharmacology·Antonio Flores-BurgessZaida Díaz-Cabiale

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