Chronic Fluoxetine Treatment Induces Maturation-Compatible Changes in the Dendritic Arbor and in Synaptic Responses in the Auditory Cortex

Frontiers in Pharmacology
Estíbaliz AmpueroUrsula Wyneken

Abstract

Fluoxetine is a selective serotonin reuptake inhibitor (SSRI) used to treat mood and anxiety disorders. Chronic treatment with this antidepressant drug is thought to favor functional recovery by promoting structural and molecular changes in several forebrain areas. At the synaptic level, chronic fluoxetine induces an increased size and density of dendritic spines and an increased ratio of GluN2A over GluN2B N-methyl-D-aspartate (NMDA) receptor subunits. The "maturation"-promoting molecular changes observed after chronic fluoxetine should also induce structural remodeling of the neuronal dendritic arbor and changes in the synaptic responses. We treated adult rats with fluoxetine (0.7 mg/kg i.p. for 28 days) and performed a morphometric analysis using Golgi stain in limbic and nonlimbic cortical areas. Then, we focused especially on the auditory cortex, where we evaluated the dendritic morphology of pyramidal neurons using a 3-dimensional reconstruction of neurons expressing mRFP after in utero electroporation. With both methodologies, a shortening and decreased complexity of the dendritic arbors was observed, which is compatible with an increased GluN2A over GluN2B ratio. Recordings of extracellular excitatory postsynaptic poten...Continue Reading

References

Mar 24, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ursula WynekenFernando Orrego
May 4, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Weiqing Ji, Nobuo Suga
Feb 14, 2008·Nature Reviews. Neuroscience·Nelson Spruston
Apr 19, 2008·Science·José Fernando Maya VetencourtLamberto Maffei
Jul 15, 2009·British Journal of Pharmacology·Laetitia MonyPierre Paoletti
Jan 29, 2010·Journal of Neurophysiology·Fernando J SepulvedaBrigitte van Zundert
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Katsunori KobayashiHidenori Suzuki
Dec 29, 2010·Hearing Research·L M Hurley, I C Hall
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 29, 2012·Journal of Neural Transmission·Peter ZwanzgerB Pfleiderer
Sep 1, 2012·Molecular Pharmacology·Stacy A Amico-RuvioGabriela K Popescu
Mar 21, 2013·Behavioural Brain Research·Estibaliz AmpueroFrancisco Javier Rubio
May 21, 2013·Nature Reviews. Neuroscience·Pierre PaolettiQiang Zhou
Feb 20, 2014·Frontiers in Systems Neuroscience·Fatima T HusainSara A Schmidt
May 3, 2014·The International Journal of Neuropsychopharmacology·Ramon GuiradoJuan Nacher
Oct 7, 2014·Frontiers in Behavioral Neuroscience·Paolo TucciLuigia Trabace
Feb 6, 2017·Journal of Cellular Physiology·Fernando J BustosBrigitte van Zundert
May 14, 2017·Progress in Neuro-psychopharmacology & Biological Psychiatry·Stefania SchiavoneMaria Grazia Morgese
Aug 22, 2017·Progress in Neuro-psychopharmacology & Biological Psychiatry·Yingying YinYonggui Yuan
Aug 22, 2017·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·L BonettiE Brattico
Dec 6, 2017·The American Journal of Psychiatry·Bret R RutherfordSteven P Roose
Feb 14, 2018·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Filippo CaraciAgata Copani
Mar 15, 2018·Molecular Brain·Qiu Jing Wu, Michael Tymianski
Jun 19, 2018·Psychological Medicine·Franco ScinicarielloPatrick N Breysse

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Citations

Aug 11, 2021·Neuropharmacology·Rodrigo Moraga-AmaroJimmy Stehberg

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