Chronic hyperosmolality increases NHE3 activity in OKP cells

The Journal of Clinical Investigation
P M AmbühlR J Alpern

Abstract

This study investigated the effect of chronic hypertonicity on the OKP cell Na/H antiporter, encoded by Na/H exchanger 3 (NHE3). Chronic (48 h) increases in extracellular glucose, mannitol, or raffinose concentration caused a significant increase in Na/H antiporter activity, while increases in urea concentration were without effect. This effect was seen with changes in osmolality of only 20 mOsm/liter, a magnitude that is observed clinically in poorly controlled diabetes mellitus. Increases in mannitol concentration acutely inhibited and chronically stimulated Na/H antiporter activity. The increase in Na/H antiporter activity induced by hypertonic incubation was resistant to 10(-7) and 5 x 10(-6) M but inhibited by 10(-4) M ethylisopropyl amiloride, consistent with regulation of NHE3. In addition, hypertonicity increased total cellular and plasma membrane NHE3 protein abundance twofold, with only a small increase in NHE3 mRNA abundance. We conclude that chronic pathophysiologically relevant increases in tonicity lead to increases in NHE3 protein abundance and activity. This may be responsible for increased proximal tubule apical membrane Na/H antiporter activity in poorly controlled diabetes mellitus, which could then contribut...Continue Reading

References

Nov 1, 1991·The Journal of Clinical Investigation·O W MoeR J Alpern
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Le BivicE Rodriguez-Boulan
Mar 1, 1989·The Journal of Membrane Biology·M SargiacomoE Rodriguez-Boulan
Mar 19, 1993·Science·J L BrewsterM C Gustin
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J F CollinsF K Ghishan
Aug 5, 1994·Science·Z Galcheva-GargovaR J Davis

❮ Previous
Next ❯

Citations

May 18, 2000·Biochemical and Biophysical Research Communications·T S ChuB S Hsieh
Oct 15, 2008·Pflügers Archiv : European journal of physiology·I Alexandru Bobulescu, Orson W Moe
Jun 12, 2004·Clinical and Experimental Pharmacology & Physiology·Deanne H HryciwPhilip Poronnik
Jun 1, 2006·Acta Physiologica·R T Alexander, S Grinstein
Jun 29, 2000·Annual Review of Biochemistry·D L Garbers, S K Dubois
Dec 17, 2009·Journal of Biomedicine & Biotechnology·Peijian He, C Chris Yun
Feb 2, 1999·The Journal of Clinical Investigation·P M AmbühlR J Alpern
Oct 31, 2006·Seminars in Nephrology·I Alexandru Bobulescu, Orson W Moe
Nov 22, 2005·Seminars in Nephrology·Giovambattista CapassoMaria Rizzo
Apr 15, 2008·Neuroscience Research·Tetsuro MorishimaKazuya Sobue
May 24, 2011·Biochemical and Biophysical Research Communications·Gabriella D Queiroz-LeiteNancy A Rebouças
Jan 16, 2007·The Journal of Biological Chemistry·R Todd AlexanderSergio Grinstein
Mar 31, 2012·American Journal of Physiology. Cell Physiology·Adriana C C Girardi, Francesca Di Sole
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva
Oct 29, 1998·The Journal of Biological Chemistry·G LamprechtC H Yun
Sep 9, 1998·The American Journal of Physiology·R L McSwineE B Chang
Jul 12, 2002·American Journal of Physiology. Renal Physiology·Andrew Baines, Patrick Ho
Jun 5, 2003·American Journal of Physiology. Endocrinology and Metabolism·S McGinnC A Pollock
Feb 8, 2000·American Journal of Physiology. Renal Physiology·M B GanzR F Reilly
Jul 18, 2001·American Journal of Physiology. Renal Physiology·E J WeinmanS Shenolikar
Jan 24, 2003·American Journal of Physiology. Cell Physiology·S McGinnC A Pollock
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Jelena KlisicPatrice M Ambühl
Jul 27, 2000·American Journal of Physiology. Cell Physiology·X YangR J Alpern
Jan 3, 2001·American Journal of Physiology. Renal Physiology·Y PengR J Alpern
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Pedro GomesPatrício Soares-da-Silva
Apr 13, 1999·The American Journal of Physiology·Y PengR J Alpern
Aug 23, 2002·Journal of the American Society of Nephrology : JASN·Valérie Besse-EschmannPatrice M Ambühl
Nov 26, 2003·Journal of the American Society of Nephrology : JASN·Jelena KlisicPatrice M Ambühl
May 17, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marcello Camici
Sep 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Begoña ZapateríaMaría Pilar Ramos-Álvarez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.