Chronic hypoxia in pregnant mice impairs the placental and fetal vascular response to acute hypercapnia in BOLD-MRI hemodynamic response imaging.

Placenta
Yehuda GinosarRinat Abramovitch

Abstract

Brief hypercapnic challenge causes acute placental hypoperfusion with fetal brain sparing on BOLD-MRI. We hypothesize that this non-invasive imaging strategy can distinguish between normal pregnancy and chronic placental hypoperfusion (using the maternal hypoxia model). Eighteen pregnant female ICR mice were randomized to three groups: normoxia, late-onset hypoxia (12%O2;E13.5-17.5) and early-onset hypoxia (12%O2;E10.5-17.5). On E17.5, animals were imaged in a 4.7-T Bruker-Biospec MRI scanner. Fast coronal True-FISP was performed to identify organs of interest (placenta and fetal heart, liver and brain). BOLD-MRI was performed at baseline and during a 4-min hypercapnic challenge (5%CO2). %-change in placental and fetal signal was analyzed from T2*-weighted gradient echo MR images. Following MRI, fetuses and placentas were harvested, weighed and immuno-stained. In normoxic mice, hypercapnia caused reduction in BOLD-MRI signal in placenta (-44% ± 7%; p < 0.0001), fetal liver (-32% ± 7%; p < 0.0001) and fetal heart (-54% ± 12%; p < 0.002), with relative fetal brain sparing (-12% ± 5%; p < 0.0001). These changes were markedly attenuated in both hypoxia groups. Baseline fetal brain/placenta SI ratio was highest in normoxic mice (1.1...Continue Reading

References

Jan 1, 1986·Biology of the Neonate·T J de GrauwW J Scott
Feb 1, 1980·American Journal of Obstetrics and Gynecology·J W Ritchie, K Lakhani
May 1, 1980·American Journal of Obstetrics and Gynecology·H P Van GeijnF P Zuspan
Mar 17, 1999·American Journal of Obstetrics and Gynecology·R O Bahado-SinghG Mari
May 4, 2000·American Journal of Physiology. Cell Physiology·R LevyD M Nelson
Jul 27, 2000·Human Brain Mapping·E MartinV L Marcar
Sep 11, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Waldemar Moll
Sep 24, 2004·BJOG : an International Journal of Obstetrics and Gynaecology·Ahmet Alexander Baschat
Sep 24, 2005·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·M J SimchenL K Hornberger
Jul 4, 2006·American Journal of Obstetrics and Gynecology·Roberta B Ness, Baha M Sibai
Jul 22, 2006·Journal of Perinatal Medicine·Riffat CheemaSaemundur Gudmundsson
Dec 1, 2007·Journal of Magnetic Resonance Imaging : JMRI·Eitan PrismanDavid Mikulis
Apr 24, 2008·Journal of Obstetrics and Gynaecology Canada : JOGC = Journal D'obstétrique Et Gynécologie Du Canada : JOGC·Derek FraserGregory A L Davies
May 30, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Margie ReamDona M Chikaraishi
Jul 17, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hila BarashRinat Abramovitch
Nov 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tracy M TomlinsonYoel Sadovsky
Mar 11, 2011·Neoplasia : an International Journal for Oncology Research·Yifat EdreiRinat Abramovitch
Aug 27, 2013·American Journal of Obstetrics and Gynecology·Sarka Lisonkova, K S Joseph
Sep 6, 2013·Journal of Magnetic Resonance Imaging : JMRI·Yifat EdreiRinat Abramovitch
Oct 27, 2015·The Journal of Physiology·Dino A Giussani
Jun 9, 2016·Translational Oncology·Terry H LandowskiNatarajan Raghunand
Sep 2, 2017·The Journal of Physiology·Lindsay S CahillJohn G Sled
Dec 23, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lindsay S CahillJohn G Sled

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