Mar 1, 1977

Chronic infection of HeLa cells with respiratory syncytial virus

Voprosy virusologii
O D Iankevich, R S Dreĭzin


Chronic infection of HeLa cells with respiratory syncytial virus (HeLa-RS) was produced at a multiplicity of infection of 0.00005 TCD50/cell. During 144 days, 21 passages of HeLa-RS culture were done. The chronically infected cells did not differ from the control culture in the growth pattern and their proliferative activity. The infectious virus was found in the culture fluid with cells at 7 and 14 days after inoculation of the culture. Subsequent attempts at isolation of the infectious virus from the culture fluid and homogenates of the infected cells were fruitless. However, the specific antigen was detected by the immunofluorescence procedure in 17.9-26.0% of cells (6-7 days after cell plating). The number of the infected cells increased after treatment of the culture with 5-iodo-2-deoxyuridine. Passage of HeLa-RS cells in the medium containing specific antibody had no effect on the number of cells containing RS-virus antigen. HeLa-RS cells were moderately resistant to superinfection with RS virus and retained their sensitivity to adenovirus type 3. Out of 9 clones examined 8 were infected. No interferon was found.

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Mentioned in this Paper

Respiratory Syncytial Virus Infections
Antibodies, Viral
Virus Diseases
Respiratory syncytial virus
HeLa Cells
M Phase, Mitotic
Idoxuridine, Radical Ion (1-)
Communicable Diseases

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