PMID: 9165291Jan 1, 1997Paper

Chronic inhibition of the high-affinity dopamine uptake system increases oxidative damage to proteins in the aged rat substantia nigra

Free Radical Biology & Medicine
M Romero-RamosA Machado

Abstract

The effect of chronic treatment of aged rats with nomifensine has been studied in the rat nigrostriatal dopaminergic system. The rat substantia nigra suffers an oxidative damage during aging that results in both an increase in carbonyl groups of its total proteins and the oxidative inactivation of tyrosine hydroxylase (TH) enzyme, which are partially reversed by chronic treatment with deprenyl. Different mechanisms may account for this effect, including inhibition of the high-affinity dopamine uptake system. We treated aged rats chronically with nomifensine for 2 months and found some significant effects. Nomifensine treatment significantly increased TH enzyme amount in substantia nigra (39.2%), which was accompanied by a significant increase in TH enzyme activity (47.8%). However, these effects were not observed in the terminal field (striatum). As a further step we quantified the oxidative level of proteins by measuring the number of carbonyl groups coupled either to total proteins or specifically to TH enzyme. The proteins of aged rat substantia nigra showed a significant increase of carbonyl groups following nomifensine treatment. The number of carbonyl groups coupled to nigral TH enzyme also increased in the nomifensine-tr...Continue Reading

References

Nov 1, 1992·Journal of Neurochemistry·B Halliwell
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M B Spina, G Cohen
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Sep 2, 1983·European Journal of Pharmacology·G Chamba, B Renaud
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D Harman
Sep 1, 1994·Journal of Neurochemistry·M GerlachM B Youdim
Jan 1, 1994·Pharmacology, Biochemistry, and Behavior·D L GelowitzC T Lai
Jan 21, 1993·The New England Journal of Medicine·UNKNOWN Parkinson Study Group
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·N A Simonian, J T Coyle
Jan 1, 1996·Free Radical Biology & Medicine·C P De La CruzA Machado

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Citations

May 31, 2006·Neurochemical Research·Donatella MarazzitiGiovanni B Cassano
Oct 22, 1998·Neurology·P MecocciU Senin

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