PMID: 9166743Jun 1, 1997Paper

Chronic intermittent ethanol treatment in rats increases GABA(A) receptor alpha4-subunit expression: possible relevance to alcohol dependence

Journal of Neurochemistry
M MahmoudiR W Olsen

Abstract

Chronic administration of ethanol to rats on an intermittent regimen, for 60 repeated intoxicating doses and repeated withdrawal episodes, results in a long-lasting kindling phenomenon. This involves an increasing severity of withdrawal, including a reduced threshold to seizures produced by the GABA(A) antagonist, pentylenetetrazol. We have shown previously that muscimol-evoked 36Cl- efflux and paired-pulse inhibition (involving GABA(A)-mediated recurrent inhibition) were decreased persistently in the CA1 region of hippocampal slices from chronic intermittent ethanol (CIE)-treated rats. We now report elevated levels of mRNA in forebrain for the alpha4 subunit of the GABA(A) receptor (GABAR), considered to be a constituent of pharmacologically and physiologically novel subtypes of GABARs. Using in situ hybridization with digoxigenin-labeled RNA probes, we show that at 2 days withdrawal, 60-dose CIE leads to a significant 30% increase in alpha4 subunit mRNA levels in the dentate gyrus, 46% increase in the CA3, and 26% increase in the CA1 regions. In contrast, there was no significant change in the mRNAs for the alpha5 subunit or glutamic acid decarboxylase 67 in the same regions. This study suggests that GABAR subunit-selective a...Continue Reading

Citations

Dec 20, 2005·Neurochemical Research·Richard W OlsenIgor Spigelman
May 23, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Rotem GenudGal Yadid
Nov 17, 2009·Biochemical Society Transactions·Hui Shen, Sheryl S Smith
Feb 17, 2015·Alcoholism, Clinical and Experimental Research·Candice E Van SkikeDouglas B Matthews
Dec 1, 2015·Frontiers in Cellular Neuroscience·Paolo FollesaGiorgio Gorini
Oct 3, 2008·Alcoholism, Clinical and Experimental Research·Tracy D StepanyanJohn M Littleton
Sep 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·D ChandraG E Homanics
Aug 31, 2013·Current Neurology and Neuroscience Reports·Henning Boecker
Mar 25, 2014·Psychopharmacology·Giovanni BiggioMariangela Serra
Sep 16, 2005·Psychopharmacology·Paolo FollesaGiovanni Biggio
Aug 19, 2015·Annals of the New York Academy of Sciences·Klaus A MiczekRosa M M de Almeida
Jul 16, 2002·Nature Neuroscience·Inger Sundstrom-PoromaaSheryl S Smith
Dec 24, 2005·Epilepsy Currents·Michael A Rogawski
Mar 4, 2003·Journal of Neurophysiology·Fu-Chun HsuSheryl S Smith
Dec 14, 2007·Alcoholism, Clinical and Experimental Research·Dev ChandraGregg E Homanics
Oct 5, 2010·British Journal of Pharmacology·Sahruh TurkmenInga-Maj Johansson
Feb 4, 2010·Pharmacological Reviews·Mikko Uusi-Oukari, Esa R Korpi
Nov 26, 2014·Alcoholism, Clinical and Experimental Research·Samuel W CentanniH S Swartzwelder
Dec 1, 2004·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Travis J Worst, Kent E Vrana

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.