Chronic intracellular infection of alfalfa nodules by Sinorhizobium meliloti requires correct lipopolysaccharide core

Proceedings of the National Academy of Sciences of the United States of America
Gordon R O CampbellGraham C Walker

Abstract

Our analyses of lipopolysaccharide mutants of Sinorhizobium meliloti offer insights into how this bacterium establishes the chronic intracellular infection of plant cells that is necessary for its nitrogen-fixing symbiosis with alfalfa. Derivatives of S. meliloti strain Rm1021 carrying an lpsB mutation are capable of colonizing curled root hairs and forming infection threads in alfalfa in a manner similar to a wild-type strain. However, developmental abnormalities occur in the bacterium and the plant at the stage when the bacteria invade the plant nodule cells. Loss-of-function lpsB mutations, which eliminate a protein of the glycosyltransferase I family, cause striking changes in the carbohydrate core of the lipopolysaccharide, including the absence of uronic acids and a 40-fold relative increase in xylose. We also found that lpsB mutants were sensitive to the cationic peptides melittin, polymyxin B, and poly-l-lysine, in a manner that paralleled that of Brucella abortus lipopolysaccharide mutants. Sensitivity to components of the plant's innate immune system may be part of the reason that this mutant is unable to properly sustain a chronic infection within the cells of its host-plant alfalfa.

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Citations

Oct 26, 2002·Trends in Plant Science·Axel Mithöfer
Jul 17, 2007·Nature Reviews. Microbiology·Kathryn M JonesGraham C Walker
Mar 26, 2003·European Journal of Biochemistry·Nicolas FraysseVéréna Poinsot
Feb 25, 2006·The Journal of Biological Chemistry·Suparna Kanjilal-KolarChristian R H Raetz
Oct 15, 2011·Molecular Plant-microbe Interactions : MPMI·Attila KeresztEva Kondorosi
Aug 12, 2010·Molecular Plant-microbe Interactions : MPMI·Michiko E Taga, Graham C Walker
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·John Beck JensenT V Bhuvaneswari
Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Jeroen Den HerderSofie Goormachtig
Jan 11, 2008·Molecular Plant-microbe Interactions : MPMI·Nataliya PobigayloAnke Becker
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Anke BeckerJacques Batut
Jul 5, 2008·Plant Physiology·Griet Den HerderSofie Goormachtig
Jan 19, 2010·Plant Physiology·Juan Carlos Pérez GuerraMarcelle Holsters
Jan 25, 2011·Applied and Environmental Microbiology·M Z Tadra-SfeirR A Monteiro
May 29, 2012·Journal of Bacteriology·Hai-Yang LuHai-Ping Cheng
Jun 12, 2013·Journal of Bacteriology·Matthew B CrookJoel S Griffitts
Jun 19, 2003·Journal of Bacteriology·Gordon R O CampbellGraham C Walker
Nov 6, 2008·Annual Review of Genetics·Katherine E GibsonGraham C Walker
Jan 1, 2010·Microbes and Environments·Ken-Ichi KuchoPhilippe Normand
Aug 2, 2013·International Journal of Molecular Sciences·Pablo C BoginoWalter Giordano
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·René MathisDanny Vereecke
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter MergaertEva Kondorosi
Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gail P FergusonGraham C Walker
Jan 20, 2016·The Journal of General and Applied Microbiology·Seiji WakaoHiroshi Oyaizu
Feb 2, 2010·Trends in Plant Science·Olivier Schumpp, William J Deakin

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