Chronic low-dose γ-irradiation of Drosophila melanogaster larvae induces gene expression changes and enhances locomotive behavior

Journal of Radiation Research
Cha Soon KimSeon Young Nam

Abstract

Although radiation effects have been extensively studied, the biological effects of low-dose radiation (LDR) are controversial. This study investigates LDR-induced alterations in locomotive behavior and gene expression profiles of Drosophila melanogaster. We measured locomotive behavior using larval pupation height and the rapid iterative negative geotaxis (RING) assay after exposure to 0.1 Gy γ-radiation (dose rate of 16.7 mGy/h). We also observed chronic LDR effects on development (pupation and eclosion rates) and longevity (life span). To identify chronic LDR effects on gene expression, we performed whole-genome expression analysis using gene-expression microarrays, and confirmed the results using quantitative real-time PCR. The pupation height of the LDR-treated group at the first larval instar was significantly higher (∼2-fold increase in PHI value, P < 0.05). The locomotive behavior of LDR-treated male flies (∼3 - 5 weeks of age) was significantly increased by 7.7%, 29% and 138%, respectively (P < 0.01), but pupation and eclosion rates and life spans were not significantly altered. Genome-wide expression analysis identified 344 genes that were differentially expressed in irradiated larvae compared with in control larvae. ...Continue Reading

References

Jan 1, 1997·Journal of the National Cancer Institute·J H Lubin, J D Boice
Feb 1, 1997·Insect Molecular Biology·S E KorochkinaH Benes
Nov 30, 2004·Gene Expression Patterns : GEP·Anita HsounaTien Hsu
May 28, 2005·Experimental Gerontology·Julia Warner GarganoMichael S Grotewiel
May 26, 2007·Radiation Research·M TubianaR Masse
Aug 22, 2007·Genome Biology·Katherine W JordanTrudy F C Mackay
Feb 26, 2008·Journal of Radiation Research·Tetsuya SakashitaYasuhiko Kobayashi
Sep 27, 2008·Developmental Biology·Svetla DimitrovaGaia Tavosanis
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 17, 2009·Biochemical and Biophysical Research Communications·Zhefeng Gong
Oct 1, 2011·Brain, Behavior, and Immunity·Jason M YorkGregory G Freund
Mar 1, 2012·Journal of Radiation Research·Ki Moon SeongYoung-Woo Jin

❮ Previous
Next ❯

Citations

Oct 21, 2017·Dose-response : a Publication of International Hormesis Society·Yuta ShibamotoNatsuto Aoyama
Aug 15, 2018·International Journal of Molecular Sciences·Yuta Shibamoto, Hironobu Nakamura
Jul 30, 2019·Journal of Toxicology and Environmental Health. Part a·Elizabeth JiménezAraceli Amaya-Chavez
Mar 29, 2019·Informatics for Health & Social Care·Blaine ReederCatherine M Jankowski
Apr 28, 2020·International Journal of Radiation Biology·Luz Ma VidalJuan C Sánchez-Meza
Dec 9, 2020·Journal of Radiation Research·Yoshiharu Tanaka, Masakazu Furuta
Aug 18, 2021·International Journal of Radiation Biology·Neha AgrawalEkta Kohli

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE61759

Methods Mentioned

BETA
feature extraction
PCR
irradiating

Software Mentioned

GSEA
Prism
GeneSpring GX
Image J

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.