Abstract
We have recently characterized two lymphocyte-associated membrane proteins which have been termed 225,000-dalton and 185,000-dalton macromolecular insoluble cold globulin (225-MICG and 185-MICG, respectively) to distinguish their major physicochemical properties. These proteins differ antigenically, structurally, and in their cellular distribution. T cells can be distinguished by the synthesis and presence in the plasma membrane of 225-MICG, Null cells by the appearance of 185-MICG, and B cells by the appearance of both 225- and 185-MICG. The characterization of these two proteins in the monoclonal B lymphocytes of chronic lymphocytic leukemia forms the basis of this report. Using immunofluorescent microscopy, we found only 225-MICG on the surface of chronic lymphocytic leukemia (CLL) cells in 15 patients, whereas control B cells from 20 individuals displayed both 225- and 185-MICG. When MICG proteins were isolated and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, normal B cells showed two stained bands, corresponding to 225- and 185-MICG, whereas the CLL cells demonstrated only the 225-MICG band. Using labeled amino acid incorporation into cellular protein, normal B cells were shown to synthesize 225- ...Continue Reading
References
Aug 26, 1978·Lancet·P J FialkowL Steinmann
Jan 1, 1979·The Journal of Experimental Medicine·S P HauptmanS Godfrey
Dec 1, 1976·The Journal of Clinical Investigation·S BroderT A Waldmann
Jan 1, 1977·Scandinavian Journal of Immunology·G JanossyT A Platts-Mills
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J L TouraineR A Good
Apr 20, 1978·The New England Journal of Medicine·L B VoglerM D Cooper
Jul 1, 1978·Immunochemistry·S P HauptmanI A Gutterman
Nov 23, 1978·Nature·S P Hauptman, E Kansu
Jun 20, 1978·Annals of the New York Academy of Sciences·B R ZetterD Gospodarowicz
Sep 1, 1978·Annals of Internal Medicine·P J RosenR J Lukes
Jul 1, 1978·Immunochemistry·S P Hauptman
Jan 1, 1976·Journal of Immunological Methods·E AldersonJ J Owen
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L B ChenJ K McDougall
Jan 1, 1975·The Journal of Clinical Investigation·H J Cohen
Oct 1, 1970·The Journal of Experimental Medicine·C BiancoV Nussenzweig
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J L Preud'homme, M Seligmann
Mar 1, 1970·The Journal of Experimental Medicine·R Laskov, M D Scharff
Jan 1, 1974·Scandinavian Journal of Immunology·J L Preud'hommeM Seligmann
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D Zucker-FranklinF Quagliata
Jan 5, 1972·Nature: New Biology·R M ParkhouseM F Greaves
Oct 1, 1973·The Journal of Experimental Medicine·D S RoweB Pernis
Aug 10, 1972·The New England Journal of Medicine·A C Aisenberg, K J Bloch
Feb 1, 1973·The Journal of Clinical Investigation·G D RossH M Grey
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J Andersson, F Melchers
Feb 1, 1974·The Journal of Experimental Medicine·S M FuH G Kunkel
Jun 1, 1974·The Journal of Experimental Medicine·E S Vitetta, J W Uhr
Mar 1, 1974·The Journal of Clinical Investigation·S Hauptman, T B Tomasi
Jul 1, 1974·The Journal of Clinical Investigation·S Hauptman, T B Tomasi
Nov 1, 1970·The Journal of Experimental Medicine·B PernisL Amante
Mar 14, 1971·Journal of Molecular Biology·R LaskovM D Scharff
Citations
Jul 30, 1982·Journal of Immunological Methods·Y TannoT Takishima
Jul 1, 1984·The Journal of Clinical Investigation·G B SegelM A Lichtman
Jan 1, 1985·Annals of the New York Academy of Sciences·N Dainiak, C M Cohen
Sep 1, 1985·Journal of Cellular Physiology·G B SegelM A Lichtman
Nov 1, 1990·Journal of Cellular Physiology·T J WoodlockR Silber