PMID: 12753083May 20, 2003Paper

Chronic nicotine administration does not increase nicotinic receptors labeled by [125I]epibatidine in adrenal gland, superior cervical ganglia, pineal or retina

Journal of Neurochemistry
Martha I Dávila-GarcíaKenneth J Kellar

Abstract

Neuronal nicotinic acetylcholine receptors (nAChRs) were measured in CNS and peripheral tissues following continuous exposure to saline or nicotine hydrogen tartrate (3.3 or 10 mg/kg/day) for 14 days via osmotic pumps. Initially, binding of [3H](-)nicotine, [3H]cytisine and [3H]epibatidine to nAChRs was compared to determine the suitability of each for these kinds of studies. The predominant nAChR labeled by agonists in the cerebral cortex is an alpha 4 beta 2 subtype, whereas the predominant nicotinic receptors in the adrenal gland, superior cervical ganglia and pineal gland contain an alpha 3 subunit, and they do not bind either [3H](-)nicotine or [3H]cytisine with high affinity. In retina some nAChRs bind all three ligands with high affinity, and others appear to bind only [3H]epibatidine. Thus, only [3H]epibatidine had high enough affinity to be useful for measuring the nAChRs in all of the tissues. The receptors from nicotine-treated rats were then measured using [125I]epibatidine, which has binding characteristics very similar to [3H]epibatidine. Treatment with the two doses of nicotine hydrogen tartrate increased binding sites in the cerebral cortex by 40% and 70%, respectively. In contrast, no significant changes in the...Continue Reading

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Citations

Jun 3, 2009·Archives of General Psychiatry·Kelly P CosgroveJulie K Staley
Feb 1, 2012·Human Genetics·Allison B Gold, Caryn Lerman
Aug 19, 2007·The Journal of Biological Chemistry·Renaldo C DrisdelWilliam N Green
Apr 26, 2012·The Journal of Pharmacology and Experimental Therapeutics·Cristian A ZambranoMichael J Marks
Nov 16, 2006·Annals of the New York Academy of Sciences·Malathi SrivatsanEzekiel E Shotts
Dec 24, 2011·Journal of Neurochemistry·Kabirullah LutfyTheodore C Friedman
Mar 3, 2009·Journal of Psychopharmacology·K A BrennanP Truman
May 3, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juan ArredondoSergei A Grando
Apr 21, 2007·The Journal of Pharmacology and Experimental Therapeutics·David C PerryKenneth J Kellar
Sep 26, 2015·Pharmacological Reviews·Esa R KorpiGavin S Dawe
Oct 16, 2003·The Journal of Pharmacology and Experimental Therapeutics·Henry N NguyenDavid C Perry
Mar 16, 2004·The Journal of Pharmacology and Experimental Therapeutics·Yingxian Xiao, Kenneth J Kellar
Jul 13, 2004·Molecular Pharmacology·Susan C HernandezKenneth J Kellar
Oct 8, 2005·The Journal of Pharmacology and Experimental Therapeutics·Maryka Quik, J Michael McIntosh

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