Chronic Stress in a Rat Model of Depression Disturbs the Glutamine-Glutamate-GABA Cycle in the Striatum, Hippocampus, and Cerebellum

Neuropsychiatric Disease and Treatment
Shaohua XuPeng Xie

Abstract

Major depressive disorder (MDD) is a complex psychiatric illness involving multiple brain regions. Increasing evidence indicates that the striatum is involved in depression, but the molecular mechanisms remain unclear. In this study, we performed a gas chromatography-mass spectrometer (GC/MS)-based metabolomic analysis in the striatum of depressed rats induced by chronic unpredictable mild stress (CUMS). We then compared striatal data with our previous data from the hippocampus and cerebellum to systematically investigate the potential pathogenesis of depression. We identified 22 differential metabolites in the striatum between the CUMS and control groups; these altered metabolites were mainly involved in amino acid, carbohydrate, and nucleotide metabolism. Pathway analysis revealed that the shared metabolic pathways of the striatum, hippocampus, and cerebellum were mainly involved in the glutamine-glutamate metabolic system. Four genes in the striatum (GS, GLS2, GLT1, and SSADH), six genes in the hippocampus (GS, SNAT1, GAD1, SSADH, VGAT, and ABAT), and five genes in the cerebellum (GS, ABAT, SNAT1, VGAT, and GDH) were found to be significantly altered using RT-qPCR. Correlation analysis indicated that these differential genes...Continue Reading

References

Jan 1, 1978·Biochemical Pharmacology·P S CalleryM Stogniew
Aug 2, 2003·Annals of Neurology·Edward J NovotnyDouglas L Rothman
Apr 2, 2005·Nature Reviews. Neuroscience·David A LewisDavid W Volk
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·P V ChoudaryE G Jones
Dec 21, 2005·The Journal of Nutrition·Yoshiharu Shimomura, Robert A Harris
Jan 21, 2006·The Journal of Nutrition·Eva Blomstrand
Jun 9, 2006·Progress in Neurobiology·Masao Ito
Aug 17, 2006·The Australian and New Zealand Journal of Psychiatry·Lisa J PhillipsGregor Berger
Sep 14, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Christopher Pittenger, Ronald S Duman
Jun 10, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Harin KananiMaria I Klapa
Oct 17, 2008·Nature·Vaishnav Krishnan, Eric J Nestler
Oct 24, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Dara M CannonWayne C Drevets
Oct 25, 2008·Nature·Jeremy K Nicholson, John C Lindon
May 5, 2009·Biological Psychiatry·Alvaro L Garcia-GarciaRosa M Tordera
Mar 20, 2010·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Shang-Feng Gao, Ai-Min Bao
Jun 10, 2010·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Anita J Bechtholt-GompfBruce M Cohen
Jan 14, 2012·Journal of Proteome Research·Peng ZhengPeng Xie
Nov 1, 2012·Molecular & Cellular Proteomics : MCP·Peng ZhengPeng Xie
Nov 28, 2012·Brain Structure & Function·Javier Gilabert-JuanJuan Nacher
Dec 27, 2012·Progress in Neuro-psychopharmacology & Biological Psychiatry·W N Marsden
Nov 7, 2013·Pflügers Archiv : European journal of physiology·Stefan Bröer
Aug 2, 2014·Journal of Affective Disorders·Matthew J Taylor
Dec 9, 2014·Journal of Pediatric Epilepsy·Mahsa ParvizPhillip L Pearl
Mar 31, 2015·Neuroscience·J DetkaB Budziszewska
Jun 2, 2015·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Danilo ArnoneJonathan Cavanagh
Nov 26, 2015·Neuroscience and Biobehavioral Reviews·Huawei ZhangQiyong Gong
Mar 1, 2016·Current Neuropharmacology·Yashika Bansal, Anurag Kuhad
Mar 30, 2016·Molecular and Cellular Biochemistry·Aza ShetewyStephen W Schaffer
May 7, 2016·Psychoneuroendocrinology·Toni Ali-SistoSoili M Lehto

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Methods Mentioned

BETA
Polymerase Chain Reaction
PCA

Software Mentioned

BLAST
SPSS
Ethovision
ChromaTOF
MetaboAnalyst
R
IPA
MetaboAnalyst4

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