PMID: 9556797Apr 29, 1998Paper

Chronic transplant reaction of the kidney. A interphase cytogenetic and immunohistologic characterization of the involved cells in relation to donor and recipient origin

Der Pathologe
Iris BittmannH Schneeberger

Abstract

Chronic rejection is a major problem in contemporary kidney transplantation. The purpose of this study was to determine whether renal cells are repopulated by extra-renal cells over time or whether the graft remains permanently allogenic. We studied nine explanted allografted kidneys of sex-mismatched donors by means of non-isotopic in situ hybridization (NISH). We used biotinylated centromer-specific DNA probes of the human chromosomes Y and X. In a further step, monoclonal and polyclonal antibodies against CD45, CD3, CD20, CD31, CD1a, S100, alpha-actin, factor Vill and UEA were used to analyse the various infiltrating cell types and the cells involved in allograft arteriopathy. In several cases NISH and immunohistochemistry were combined to facilitate the typing of cells. Our study showed that up to several years after transplantation the glomerular, tubular and endothelial cells retained donor origin. The only cells of recipient origin were the inflammatory cells, predominantly macrophages and T lymphocytes.

Citations

Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wolfram KleebergerHans Kreipe
Mar 6, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H RienstraJ L Hillebrands

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