Chronic treatment in vivo with dimethylthiourea, a hydroxyl radical scavenger, prevents diabetes-induced endothelial dysfunction

Journal of Cardiovascular Pharmacology
G M PieperM B Adams

Abstract

Oxidative stress is believed to play a role in diabetes-induced vascular complications. In this study, we tested whether chronic treatment with a known hydroxyl radical scavenger, dimethylthiourea (DMTU), could prevent endothelial dysfunction in diabetes. Lewis strain rats were made diabetic by an intravenous injection of streptozotocin. A subgroup of diabetic animals received daily intraperitoneal injections of 50 mg/kg DMTU beginning at 72 h after streptozotocin and throughout 8 weeks of diabetes. Diabetes caused an increase in aortic catalase activity (an index of compensatory in vivo oxidative stress) that was not prevented by long-term DMTU treatment. Long-term treatment of diabetic animals with DMTU did not alter serum insulin levels, blood glucose concentrations, or total glycosylated hemoglobin. Descending thoracic aortas were isolated, sectioned into rings and suspended in isolated tissue baths, and contracted with a submaximal concentration of norepinephrine. Relaxation to the endothelium-dependent vasodilator, acetylcholine, was impaired in diabetic aortas, whereas relaxation to A23187 and nitroglycerin was unaltered. DMTU treatment prevented the diabetes-induced impairment in endothelium-dependent relaxation to acet...Continue Reading

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Citations

May 29, 2002·Free Radical Biology & Medicine·Galen M PieperPiero Del Soldato
Mar 17, 2000·Free Radical Biology & Medicine·M Qian, J W Eaton
Oct 7, 2004·European Journal of Pharmacology·Matthew R NangleNorman E Cameron
Mar 10, 2004·The Journal of Pharmacy and Pharmacology·G Ozansoy, F B Akin
Jun 13, 2014·The Journal of Physical Chemistry. a·Tabitha ChigwadaReuben H Simoyi
Aug 29, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M WeihT Grune
Jun 9, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·M D BoudreauJay C Means
Jul 24, 1998·Journal of Cardiovascular Pharmacology·G M Pieper, W Siebeneich
Jul 18, 2017·BioMed Research International·Nahed El-NajjarKhaled Machaca
Jul 28, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Dong-Feng YeihYung-Zu Tseng
Dec 7, 2010·The Analyst·Mária KatonaZoltán Takáts

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