Chronic treatment with atrial natriuretic peptide in spontaneously hypertensive rats: beneficial renal effects and sex differences

PloS One
Mariana RomeroAnalía L Tomat

Abstract

The aim of this study was to investigate the effects of chronic treatment with atrial natriuretic peptide (ANP) on renal function, nitric oxide (NO) system, oxidative stress, collagen content and apoptosis in kidneys of spontaneously hypertensive rats (SHR), as well as sex-related differences in the response to the treatment. 10 week-old male and female SHR were infused with ANP (100 ng/h/rat) or saline (NaCl 0.9%) for 14 days (subcutaneous osmotic pumps). Systolic blood pressure (SBP) was recorded and diuresis and natriuresis were determined. After treatment, renal NO synthase (NOS) activity and eNOS expression were evaluated. Thiobarbituric acid-reactive substances (TBARS), glutathione concentration and glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in the kidney. Collagen was identified in renal slices by Sirius red staining and apoptosis by Tunel assay. Female SHR showed lower SBP, oxidative stress, collagen content and apoptosis in kidney, and higher renal NOS activity and eNOS protein content, than males. ANP lowered SBP, increased diuresis, natriuresis, renal NOS activity and eNOS expression in both sexes. Renal response to ANP was more marked in females than in males. In kidney, A...Continue Reading

References

Jan 1, 1978·Biochemical Pharmacology·Y Masuda, T Murano
Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
Dec 4, 1986·Journal of Immunological Methods·K TaketaT Hanada
Jan 1, 1984·Methods in Enzymology·L Flohé, W A Günzler
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·I KishimotoD L Garbers
Oct 1, 1995·American Journal of Hypertension·N WiinbergM W Bentzon
Feb 1, 2000·Biochemical and Biophysical Research Communications·T PelzerL Neyses
Aug 15, 2000·Journal of Cardiovascular Pharmacology·S YangL Zhang
May 23, 2001·Hypertension·J F Reckelhoff
Sep 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·G S FilippatosB D Uhal
Sep 18, 2001·Heart·R D Rakhit, M S Marber
Feb 28, 2002·Cardiovascular Research·Raghvendra K DubeyEdwin K Jackson
May 9, 2002·American Journal of Physiology. Cell Physiology·Wojciech NowakWilliam S Spielman
Jun 1, 2002·Circulation Research·Gary PareMichael E Mendelsohn
Jun 18, 2002·Journal of Cellular and Molecular Medicine·A OrtizJ Egido
Jul 19, 2002·Cardiovascular Research·Tohru FukaiDavid G Harrison
Dec 14, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Balazs AntusLaszlo Rosivall
Jan 1, 1954·Methods of Biochemical Analysis·A C MAEHLY, B CHANCE
Jan 21, 2004·Cardiovascular Research·Ana Paula V DantasMaria Helena C Carvalho
Mar 9, 2004·Regulatory Peptides·María de los Angeles CostaCristina Arranz
Apr 20, 2004·Current Opinion in Nephrology and Hypertension·Nosratola D Vaziri
Sep 27, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher S Wilcox
Aug 19, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jennifer C SullivanJennifer S Pollock
Feb 16, 2007·Life Sciences·Analía Lorena TomatCristina Teresa Arranz
Jun 26, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Makoto ShonoHisao Ogawa
Mar 22, 2008·Hypertension·John E HallKathryn Sandberg
Jun 6, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Analía Lorena TomatCristina Arranz

❮ Previous
Next ❯

Citations

May 1, 2020·Antioxidants & Redox Signaling·Jian YangChunyu Zeng
Feb 20, 2019·International Journal of Molecular Sciences·Speranza RubattuMassimo Volpe
Jun 9, 2021·The Journal of Clinical Investigation·Soon Y TangGarret A FitzGerald

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Totallab analyzer
Graph Pad
Prism
Image
Pro Plus
PowerLab

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis