Chronic treatment with estrogen up-regulates expression of sst2 messenger ribonucleic acid (mRNA) but down-regulates expression of sst5 mRNA in rat pituitaries

Endocrinology
N KimuraN Kimura

Abstract

We previously showed that 17beta-estradiol (E2) induces the somatostatin (SRIF) responsiveness of the rat anterior pituitary, which leads to inhibition of PRL secretion through E2-dependent SRIF receptors. To examine receptor subtypes regulated by E2, we determined the messenger ribonucleic acid (mRNA) levels of all subtypes using a semiquantitative RT-PCR and characterized pituitary membrane receptors using subtype-preferential SRIF analogs. Most of the SRIF receptor subtype mRNAs were sst5 and sst2A in ovariectomized rat pituitaries [sst5/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) = 1.4 x 10(-2), sst2A/GAPDH = 0.4 x 10(-2)]. The expression pattern of the subtypes in ovariectomized rat pituitaries was similar to that of normal male and female rat pituitaries, although the mRNA levels of sst5 and sst2A in male rat pituitaries were higher than in females. Chronic administration (4 weeks) of E2 to the ovariectomized rats increased mRNA expression of sst2A, sst2B, sst3, and sst1 and drastically decreased expression of sst5; the transcripts of sst2 isoforms constituted 87% of total SRIF receptor subtype mRNAs (sst2A/GAPDH = 1.2 x 10(-2)), whereas the sst5 mRNA level was less than 1%. Receptor-binding studies revealed that in ...Continue Reading

Citations

May 28, 2009·American Journal of Physiology. Endocrinology and Metabolism·Johannes D VeldhuisCyril Y Bowers
Oct 15, 2010·American Journal of Physiology. Endocrinology and Metabolism·José Córdoba-ChacónRaul M Luque
Aug 16, 2008·European Journal of Endocrinology·Johannes D Veldhuis, Daniel M Keenan
Mar 19, 2004·European Journal of Nuclear Medicine and Molecular Imaging·B Van Den BosscheC Van de Wiele
Mar 15, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Catalina NormanJohannes D Veldhuis
May 22, 2002·Endocrinology·Whitney W WoodmanseeE Chester Ridgway
Feb 18, 2003·Endocrine Reviews·Leo J Hofland, Steven W J Lamberts
Aug 10, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Johannes D VeldhuisCyril Y Bowers
Jun 29, 2006·The Journal of Clinical Endocrinology and Metabolism·Johannes D VeldhuisCyril Y Bowers
Jan 26, 2006·Endocrine Reviews·Johannes D VeldhuisCyril Y Bowers
Feb 3, 2018·Journal of Pediatric Endocrinology & Metabolism : JPEM·Folakemi O Irewole-OjoOlisamedua F Njokanma
Dec 13, 2005·Experimental Biology and Medicine·Charles A BlakeSteve M Helmke
May 7, 2010·The Journal of Clinical Endocrinology and Metabolism·Vita BirznieceKen K Y Ho
Aug 6, 1999·Frontiers in Neuroendocrinology·Y C Patel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.