Chronically HIV-1 Infected Patients Exhibit Low Frequencies of CD25+ Regulatory T Cells.

The Open Virology Journal
Cesar Mauricio Rueda RiosMaria Teresa Rugeles

Abstract

The characterization of regulatory T cells (Treg) during HIV infection has become of particular interest considering their potential role in the pathogenesis of the acquired immunodeficiency syndrome. Different reports on Tregs in HIV-infected patients vary greatly, depending on the state of disease progression, anatomical compartment, and the phenotypic markers used to define this cell subpopulation. To determine the frequency of Tregs we included paired samples from peripheral blood and rectal biopsies from controls and chronic HIV patients with or without detectable viral load. Tregs were determined by flow cytometry using three different protocols: CD4(+)Foxp3(+); CD4(+)Foxp3(+)CD127(Low/-), and CD4(+)CD25(+)CD127(Low/-). In addition, and with the purpose to compare the different protocols we also characterized Tregs in peripheral blood of HIV negative individuals with influenza like symptoms. Here, we report that Treg characterization in HIV-infected patients as CD4(+)Foxp3(+) and CD4(+)Foxp3(+)CD127(Low/-) cells was similar, indicating that both protocols represent a suitable method to determine the frequency of Tregs in peripheral blood mononuclear cells (PBMC) and gut associated lymphoid tissue (GALT). In contrast, in H...Continue Reading

Citations

Nov 17, 2015·Journal of Immunology Research·Jacqueline María Valverde-VillegasJosé Artur Bogo Chies
Apr 29, 2016·Clinical and Experimental Immunology·A L Rodríguez-PereaP A Velilla
Sep 13, 2019·AIDS Research and Human Retroviruses·Farouk Abou HassanNada M Melhem

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Methods Mentioned

BETA
flow cytometry
biopsy
FCS
biopsies

Software Mentioned

GraphPad Prism
Kaluza

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