Chronological occurrence of PI3KCA mutations in breast cancer liver metastases after repeat partial liver resection

BMC Cancer
Aldrick RuizAntoinette Lemoine

Abstract

Liver metastases of breast cancer are frequent and can recur even after "complete/R0" resection in combination with systemic and hormonal treatments. The aim of this study was to analyze throughout repeat hepatectomies for liver metastases the evolution of PI3KCA gene mutational status. All liver metastases nodules (n = 70) from 19 women who underwent at least 2 liver resections were reexamined. DNA extraction from archived tumoral tissue was performed and the major 'hot spot' mutations in the helical and catalytic domains of PI3KCA have been analyzed using Massarray platform (Agena Bioscience) based on allelic discrimination PCR amplification followed by sensitive mass spectrometry detection. The two major somatic hot spot PI3KCA mutations were found in 27 (38.6%) nodules corresponding to 8 of the 19 patients (42%). The frequency of women whose breast cancer liver metastases (BCLM) carries PI3KCA mutations increased from the first to the third hepatectomy. Tumors carrying PI3KCA mutations are significantly larger and more frequently observed when resections were R0 compared to patients with no PI3KCA mutation. PI3KCA mutations are frequently observed in BCLM and persist along with the recurrence. Their identification in circul...Continue Reading

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Citations

Jul 23, 2020·Frontiers in Oncology·Zhonghua TaoXichun Hu

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Methods Mentioned

BETA
PCR
chip

Software Mentioned

SPSS
MassArrayTyper Analyzer
Massarray

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