Chymopapain-induced reduction of proinflammatory phospholipase A2 activity and amelioration of neuropathic behavioral changes in an in vivo model of acute sciatica

Journal of Neurosurgery
P D SawinS A Moore

Abstract

The mechanism of action underlying chymopapain (Chymodiactin) chemonucleolysis remains obscure. Radiographic studies suggest that chymopapain does not alter disc fragment size acutely; nonetheless, patients often report symptom resolution within a few days, even hours, of treatment. The authors postulate that, in addition to its chemonucleolytic action, chymopapain may possess antiinflammatory properties. To test this hypothesis, the authors assessed the ability of chymopapain to modulate the activity of the proinflammatory enzyme phospholipase A2 (PLA2) and to ameliorate behavioral changes associated with inflammatory neuropathy in an in vivo model of sciatica. Thirty-nine male Fischer rats were randomly assigned to one of three treatment groups: 1) saline, 2) betamethasone, or 3) chymopapain. All of the rats underwent unilateral sciatic nerve ligation with loose chromic gut suture to induce inflammatory mononeuropathy. The animals were tested for thermal and mechanical hyperalgesia on Days 0 (preoperation), 7 (pretreatment), and 14 (prior to death). Three animals were killed on Day 0 to determine the baseline PLA2 activity within unmanipulated rat sciatic nerves. On Day 7, three animals from each group were killed to assess P...Continue Reading

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Citations

Jan 30, 2004·The Spine Journal : Official Journal of the North American Spine Society·Conor W O'NeillJeffrey C Lotz
Jun 4, 2010·European Journal of Anaesthesiology·Oonagh T HickeyGeorge D Shorten
Feb 27, 2003·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Ralf Dirk RothoerlAlexander Brawanski
Jul 17, 2007·The Spine Journal : Official Journal of the North American Spine Society·Kee-Won RhyuJeffrey C Lotz

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Methods Mentioned

BETA
light microscopy

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