Cia5d regulates a new fibroblast-like synoviocyte invasion-associated gene expression signature.

Arthritis Research & Therapy
Teresina LaragionePércio S Gulko

Abstract

The in vitro invasive properties of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs) have been shown to correlate with disease severity and radiographic damage. We recently determined that FLSs obtained from pristane-induced arthritis (PIA)-susceptible DA rats are also highly invasive in the same in vitro assay through Matrigel. The transfer of alleles derived from the arthritis-resistant F344 strain at the arthritis severity locus Cia5d (RNO10), as in DA.F344(Cia5d) congenics, was enough to significantly and specifically reduce the invasive properties of FLSs. This genetically controlled difference in FLS invasion involves increased production of soluble membrane-type 1 matrix metalloproteinase (MMP) by DA, and is dependent on increased activation of MMP-2. In the present study we aimed to characterize the pattern of gene expression that correlates with differences in invasion in order to identify pathways regulated by the Cia5d locus. Synovial tissues were collected from DA and DA.F344(Cia5d) rats 21 days after the induction of PIA. Tissues were digested and FLSs isolated. After a minimum of four passages, FLSs were plated on Matrigel-covered dishes at similar densities, followed by RNA extraction. Illumina RatRe...Continue Reading

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Citations

Jun 3, 2010·Molecular Medicine·Teresina Laragione, Pércio S Gulko
Aug 4, 2011·Arthritis and Rheumatism·Teresina LaragionePércio S Gulko
Apr 15, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Teresina LaragionePércio S Gulko
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Jun 2, 2017·Arthritis Research & Therapy·Marta F BustamanteMonica Guma
Feb 7, 2021·Auto- Immunity Highlights·Mohammad Javad MousaviMahdi Mahmoudi
May 11, 2021·BioTechniques·Roberta FerrettiMaria Vinci
Jun 15, 2021·International Immunopharmacology·Zheng Jin, Zhenhua Zhu

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Methods Mentioned

BETA
flow cytometry
in vitro transcription
PCR
gene trap
ubiquitination

Software Mentioned

Illumina BeadStudio
CLUSTER
TREEVIEW
BD Cell - [UNK] Pro
R
Sequence Detection System

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