Cigarette smoke-induced Egr-1 represses T beta R-II expression in human skin dermal fibroblasts

Toxicology
Ji Na KimSang Wook Son

Abstract

Tobacco smoking is one of the many factors that contribute to premature skin aging, but the exact mechanism by which smoking induces facial wrinkling is still poorly understood. To investigate the regulatory potential of early growth response-1 (Egr-1) on the premature skin aging by smoking, this study examined the hypothesis that cigarette smoke-induced Egr-1 represses T beta R-II expression in human skin dermal fibroblasts (HSDFs). The protein and mRNA expressions of Egr-1 and T beta R-II were detected using Western blot and real-time RTPCR in HSDFs after exposure to cigarette smoke extract (CSE). Egr-1 and T beta R-II promoter activities were analyzed in CSE-exposed fibroblasts using luciferase assay. T beta R-II promoter activity was also evaluated in HSDFs to be transfected with Egr-1 overexpression vector. To investigative Egr-1-specific effects, we utilized Egr-1 small interfering RNA (siRNA) to inhibit Egr-1 expression. The expressions of Egr-1 protein and mRNA were increased in a time and dose-dependent manner. CSE also induced Egr-1 at the transcription level. Egr-1 was induced though phosphorylation of Erk1/2 after CSE exposure in HSDFs. We also observed the immunostained Egr-1 proteins were mainly localized from the...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C V MelloD F Clayton
May 15, 1991·Annals of Internal Medicine·D P KadunceJ J Zone
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P LemaireP Charnay
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B A ChristyD Nathans
Dec 1, 1971·Annals of Internal Medicine·H W Daniell
Feb 1, 1995·Obstetrics and Gynecology·D GradyD Petitti
Jan 1, 1995·American Journal of Public Health·V L ErnsterK Kerlikowske
Oct 27, 1998·Clinics in Dermatology·C Francès
Jun 3, 2000·Archives of Dermatological Research·L YinT Tsuji
Apr 6, 2001·Lancet·C LahmannA R Young
Feb 14, 2002·The Journal of Investigative Dermatology·Franck Verrecchia, Alain Mauviel
Sep 19, 2002·Molecular Reproduction and Development·Phillip J WilderAngie Rizzino
Jan 9, 2004·Experimental Lung Research·Takeo IshiiTakeshi Matsuse
Feb 6, 2004·Experimental Dermatology·Lei YinTakuo Tsuji
Jul 1, 2005·Journal of Cutaneous Medicine and Surgery·Anatoli FreimanGilles J Lauzon
Apr 8, 2006·American Journal of Respiratory Cell and Molecular Biology·Paul R ReynoldsJohn R Hoidal
Nov 14, 2006·American Journal of Respiratory Cell and Molecular Biology·Wen NingAugustine M K Choi
Dec 19, 2007·Archives of Dermatology·Daven N DoshiKevin D Cooper
Apr 9, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paul R ReynoldsJohn R Hoidal

❮ Previous
Next ❯

Citations

Sep 5, 2013·Journal of Dermatological Science·Sang Hoon JeongSang Wook Son

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.