Ciglitizone and 15d PGJ2 induce apoptosis in Jurkat and Raji cells

International Immunopharmacology
C C KanunfreR Curi

Abstract

Several studies have shown that PPARgamma agonists play a role in the regulation of lymphocytes function and apoptosis. However, the molecular mechanism(s) underlying the immunomodulatory effects of PPARgamma agonists are not defined yet. In this study, the effects of PPARgamma (15d PGJ2 and ciglitizone) ligands on proliferation, cytokine production and apoptosis of Jurkat and Raji cells (human T and B lymphocytes, respectively) were examined. Ciglitizone and 15d PGJ2 presented antiproliferative and cytotoxic effects on Jurkat and Raji cells as shown by [14C]-thymidine incorporation and cell viability assay. In addition, 15d PGJ2 inhibited cytokine production (IL-2 in Jurkat cells and IL-10 in Raji cells). The mechanism whereby PPARgamma agonists induced cytotoxicity is via apoptosis as shown by DNA fragmentation, nuclear condensation and phosphatidylserine externalization. The induction of apoptosis by ciglitizone and 15d PGJ2 on Jurkat and Raji cells may explain the suppression of cytokine production and the decrease in proliferation observed in both cell types. The apoptotic process was associated with a decrease in mitochondrial membrane potential and a marked down-regulation of the c-myc expression. These findings might pl...Continue Reading

References

Jan 1, 1996·Genes & Development·E White
Apr 28, 1997·Biochemical and Biophysical Research Communications·S C HsiehC L Yu
Apr 29, 1998·Annual Review of Physiology·G KroemerM Resche-Rigon
Jan 23, 1999·Oncogene·B Hoffman, D A Liebermann
May 26, 2001·The Journal of Endocrinology·P DeleriveB Staels
Jun 21, 2001·Molecular and Cellular Biology·E L SoucieL Z Penn
Mar 30, 2002·Molecular Endocrinology·Taro E AkiyamaFrank J Gonzalez
May 4, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Josué PadillaRichard P Phipps
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Diane R WonseyChi V Dang
Sep 28, 2002·International Immunopharmacology·Xia Zhang, Howard A Young
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Stefano LauroraGiuseppina Barrera

❮ Previous
Next ❯

Citations

Jul 25, 2006·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Maria Fernanda Cury-BoaventuraRui Curi
May 2, 2006·Prostaglandins & Other Lipid Mediators·Philip FunovicsAlicja Jozkowicz
Apr 29, 2011·Cancer Biology & Therapy·Tunde O Akinyeke, LaMonica V Stewart
Feb 28, 2007·Molecular Pharmacology·Mathias SollerAndreas von Knethen
Jun 9, 2007·Clinical Science·Thais Martins de LimaRui Curi

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis