Abstract
Adenovirus (Ads) is one of the most popular vectors used in gene therapy for the treatment of cancer. However, systemic therapy is limited by circulating antiviral antibodies and poor viral delivery in vivo. In this study, we used cytokine-induced killer (CIK) cells as delivery vehicles of Ads KGHV500 carrying the anti-p21Ras scFv gene to treat Ras gene-related lung cancer and investigate the anti-tumor effect in vitro and in vivo. The human lung cancer cell line A549 was employed to investigate the anti-tumor activity of recombinant Ads KGHV500 harboring the anti-p21Ras scFv gene using MTT, wound healing, transwell invasion, and apoptosis assays in vitro. Next, CIK cells were used as delivery vehicles to deliver KGHV500 carrying the anti-p21Ras scFv gene to treat A549-transplanted tumors in nude mice, and viral replication, p21Ras scFv expression, and the therapeutic efficacy were assessed. In vitro studies showed that KGHV500 had potent anti-tumor activity. In addition, in vivo, this combination therapy significantly inhibited the growth of lung cancer xenografts compared with mice treated with KGHV500 alone. KGHV500 and anti-p21Ras scFv were observed in tumor tissue, but were nearly undetectable in normal tissues. The co-del...Continue Reading
References
Jun 10, 1982·Nature·L F ParadaR A Weinberg
Jan 1, 1993·Annual Review of Biochemistry·D R Lowy, B M Willumsen
Apr 16, 1998·Gene Therapy·S FinkeI G Schmidt-Wolf
Apr 27, 2001·Nature·L JohnsonT Jacks
Aug 30, 2001·Gene Therapy·M Köping-HöggårdP Artursson
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Dec 26, 2003·The American Journal of Pathology·Koji OkudelaHitoshi Kitamura
Jan 1, 2005·American Journal of Respiratory Cell and Molecular Biology·Min QinRaj K Batra
Jun 18, 2005·Cell·Carla F Bender KimTyler Jacks
Mar 25, 2006·Science·Steve H ThorneChristopher H Contag
Sep 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Vicki KeedyDavid P Carbone
Jun 29, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L CrinòUNKNOWN ESMO Guidelines Working Group
Dec 22, 2010·ACS Chemical Biology·Johannes H Urban, Christoph A Merten
Jan 12, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·C Kent OsborneMark Sellers
Apr 1, 2011·Journal of Virology·Philomena OstapchukPatrick Hearing
May 5, 2012·Cancer Gene Therapy·B ThaciM S Lesniak
May 5, 2012·PloS One·Charles LuJack A Roth
Oct 3, 2012·PloS One·Zhi YangCheng Qian
May 18, 2013·Cancer Science·Jun KimuraItaru Endo
Jun 4, 2013·The New England Journal of Medicine·Alice T ShawPasi A Jänne
Sep 21, 2013·Onkologie·Jens Köhler, Martin Schuler
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Feb 17, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Yue YanLi Zhang
May 28, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robert H I AndtbackaRobert S Coffin
Dec 9, 2015·Annual Review of Biomedical Engineering·Melissa A KottermanDavid V Schaffer
Jan 20, 2016·International Journal of Oncology·Ju-Lun YangYing Luo
Feb 22, 2016·BMC Cancer·Ju-Lun YangQiang Feng
May 4, 2016·Current Clinical Pharmacology·Francesca CasaluceCesare Gridelli
Aug 3, 2016·Critical Reviews in Oncogenesis·Humberto Lara-Guerra, Jack A Roth
Nov 12, 2016·Gene Therapy·X Y PanJ L Yang
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Citations
Jul 16, 2020·Immunotherapy·Peng WangJulun Yang
Feb 5, 2021·Experimental Biology and Medicine·Jing QianJu-Lun Yang
Mar 1, 2020·Drug Discovery Today·Yunfeng LinLei Zhang
Aug 17, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Abdolreza OsaliAbdolreza Esmaeilzadeh
Aug 3, 2021·Anti-cancer Drugs·Yu DuJulun Yang