Cilia- and Flagella-Associated Protein 69 Regulates Olfactory Transduction Kinetics in Mice

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Anna K TalagaHaiqing Zhao

Abstract

Animals detect odorous chemicals through specialized olfactory sensory neurons (OSNs) that transduce odorants into neural electrical signals. We identified a novel and evolutionarily conserved protein, cilia- and flagella-associated protein 69 (CFAP69), in mice that regulates olfactory transduction kinetics. In the olfactory epithelium, CFAP69 is enriched in OSN cilia, where olfactory transduction occurs. Bioinformatic analysis suggests that a large portion of CFAP69 can form Armadillo-type α-helical repeats, which may mediate protein-protein interactions. OSNs lacking CFAP69, remarkably, displayed faster kinetics in both the on and off phases of electrophysiological responses at both the neuronal ensemble level as observed by electroolfactogram and the single-cell level as observed by single-cell suction pipette recordings. In single-cell analysis, OSNs lacking CFAP69 showed faster response integration and were able to fire APs more faithfully to repeated odor stimuli. Furthermore, both male and female mutant mice that specifically lack CFAP69 in OSNs exhibited attenuated performance in a buried food pellet test when a background of the same odor to the food pellet was present even though they should have better temporal resol...Continue Reading

Citations

Jan 18, 2020·Human Genetics·Aminata TouréCharles Coutton
Mar 15, 2019·Basic and Clinical Andrology·Jean-Fabrice Nsota MbangoAminata Touré
Oct 20, 2020·Chemical Senses·Timothy S McClintockJeffrey R Martens
Apr 4, 2021·International Journal of Molecular Sciences·Zuzanna SamselEwa Joachimiak
May 4, 2021·PloS One·Johannes ReisertAlan Gelperin
Mar 28, 2021·Journal of Cell Science·Kirill UkhanovJeffrey R Martens
Jun 5, 2021·Scientific Reports·Ewa JoachimiakDorota Wloga
Aug 11, 2021·Journal of Cell Science·Kirill UkhanovJeffrey R Martens
Sep 1, 2020·ACS Chemical Biology·Sangram GoreTimothy M Dore

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