CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia.

ERJ Open Research
Pedro SampaioSusana S Lopes

Abstract

Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers. Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients. The correlation coefficient between the two methods was R2=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18-9.17 Hz at 25°C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93 Hz and in the PCD group (n=...Continue Reading

Associated Software

Aug 29, 2017·Mónica Roxo-RosaPedro Sampaio
Aug 29, 2017·Mónica Roxo-RosaMónica Ferro Da Silva

References

Dec 5, 1997·The European Respiratory Journal·A Ellerman, H Bisgaard
Jul 17, 1998·The Laryngoscope·M JorissenB Van der Schueren
Nov 17, 1998·The European Respiratory Journal·A BushJ O Warner
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·F DupuitE Puchelle
Apr 3, 2001·American Journal of Respiratory and Critical Care Medicine·J C HoK W Tsang
May 19, 2001·The Laryngoscope·P MerkusP F Schouwenburg
Jul 31, 2003·Journal of Microscopy·J H SissonT A Wyatt
Apr 21, 2004·Archives of Otolaryngology--head & Neck Surgery·Thiemo HofmannGerald Wolf
Oct 6, 2005·The European Respiratory Journal·M SvartengrenK Philipson
May 26, 2006·Annual Review of Genomics and Human Genetics·Jose L BadanoNicholas Katsanis
Apr 3, 2008·The Journal of Pharmacy and Pharmacology·Roel MallantsPatrick Augustijns
Sep 2, 2009·Archives of Disease in Childhood·C O'CallaghanE Moya
Oct 21, 2009·The European Respiratory Journal·J F PaponE Escudier
Nov 17, 2009·American Journal of Respiratory and Critical Care Medicine·Wendy A StannardChris O'Callaghan
Jun 28, 2011·The Journal of Biological Chemistry·Jianfeng LinDaniela Nicastro
Sep 3, 2011·Healthcare Informatics Research·Woojae KimRae Woong Park
Mar 7, 2012·American Journal of Rhinology & Allergy·David GudisNoam A Cohen
Oct 13, 2012·Orphanet Journal of Rare Diseases·Jean-François PaponBruno Louis
Feb 4, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·K L BaileyT A Wyatt
Apr 29, 2014·Archives of Disease in Childhood·Jane S LucasUNKNOWN National PCD Service, UK
Sep 5, 2014·The European Respiratory Journal·Johanna RaidtClaudius Werner
Jun 26, 2015·Biochemia Medica·Davide Giavarina
Aug 8, 2015·Science Translational Medicine·Shannon P QuinnS Chakra Chennubhotla
Sep 30, 2015·Pediatric Pulmonology·Adam J ShapiroUNKNOWN Genetic Disorders of Mucociliary Clearance Consortium
Oct 20, 2015·Paediatric Respiratory Reviews·Amjad HoraniSteven L Brody
Feb 27, 2016·The European Respiratory Journal·Laura BehanJane S Lucas
Apr 7, 2016·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Jason J ChenBrian J F Wong
Nov 12, 2016·The European Respiratory Journal·Jane S LucasClaudia E Kuehni
Sep 8, 2017·European Respiratory Review : an Official Journal of the European Respiratory Society·Bruna Rubbo, Jane S Lucas
Oct 17, 2017·The Application of Clinical Genetics·Nadirah DamsehRaymond H Kim
Jun 16, 2018·American Journal of Respiratory and Critical Care Medicine·Adam J ShapiroUNKNOWN American Thoracic Society Assembly on Pediatrics
Mar 4, 2019·Chest·Bruna RubboUNKNOWN National PCD Service, UK
Jun 15, 2019·Orphanet Journal of Rare Diseases·Panayiotis KouisPanayiotis K Yiallouros
Nov 30, 2019·Cellular and Molecular Life Sciences : CMLS·Anu SironenHannah M Mitchison
Dec 28, 2019·Journal of Medical Genetics·Mahmoud R FassadHannah M Mitchison
Feb 16, 2020·The European Respiratory Journal·Amelia ShoemarkUNKNOWN representing the BEAT-PCD Network Guideline Development Group

❮ Previous
Next ❯

Citations

Aug 28, 2021·International Journal of Molecular Sciences·Andreia L PintoSusana S Lopes

❮ Previous
Next ❯

Software Mentioned

Excel
CiliarMove
Windows
ciliaFA
Visual Studio Express
SAVA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Respiratory and Critical Care Medicine
Wendy A StannardC O'Callaghan
American Journal of Respiratory and Critical Care Medicine
Adam J ShapiroKathryn Briones
© 2021 Meta ULC. All rights reserved