Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin share a conserved binding site on the ciliary neurotrophic factor receptor alpha chain.

The Journal of Biological Chemistry
Francois RousseauH Gascan

Abstract

Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin are members of the four-helix bundle cytokine family. These proteins signal through a common tripartite receptor composed of leukemia inhibitory factor receptor, gp130, and ciliary neurotrophic factor receptor alpha. Binding to ciliary neurotrophic factor receptor alpha occurs through an interaction site located at the C terminus of the cytokine AB loop and alphaD helix, known as site 1. In the present study, we have generated a model of neuropoietin and identified a conserved binding site for the three cytokines interacting with ciliary neurotrophic factor receptor alpha. To identify the counterpart of this site on ciliary neurotrophic factor receptor alpha, its cytokine binding domain was modeled, and the physicochemical properties of its surface were analyzed. This analysis revealed an area displaying properties complementary to the site 1 of ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin. Based on our computational predictions, residues were selected for their potential involvement in the ciliary neurotrophic factor receptor alpha binding epitope, and site-directed mutagenesis was carried out. Biochemical, cell proliferati...Continue Reading

References

Jul 5, 1991·Science·S DavisG D Yancopoulos
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J F Bazan
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D EisenbergT C Terwilliger
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jan 1, 1997·Annual Review of Immunology·T Taga, T Kishimoto
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·O DevergneE Kieff
Feb 28, 1998·Biochimie·G DeléageC Geourjon
May 2, 1998·The EMBO Journal·J BravoE Y Jones
Aug 26, 1998·The Biochemical Journal·P C HeinrichL Graeve
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·G SenaldiM S Chang
Jun 3, 2000·The EMBO Journal·J Bravo, J K Heath
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Buyong MaRuth Nussinov
Jun 28, 2003·Science·Martin J BoulangerK Christopher Garcia
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Adrian A CanutescuRoland L Dunbrack
Oct 7, 2003·Molecular Cell·Martin J BoulangerK Christopher Garcia
Feb 19, 2004·Nutrition Reviews·Emily Duff, Clifton A Baile
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Damien DerouetSylvie Chevalier
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Oct 27, 2004·Advances in Protein Chemistry·Martin J Boulanger, K Christopher Garcia
Jun 28, 2005·Proteins·Diana Mustard, David W Ritchie

❮ Previous
Next ❯

Citations

May 10, 2011·Immunologic Research·Lindsay L Jones, Dario A A Vignali
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Francois RousseauHugues Gascan
Mar 13, 2014·Orphanet Journal of Rare Diseases·Dawn MikelonisJulia Thom Oxford
May 4, 2011·Stem Cell Reviews and Reports·Marie-Emmanuelle MathieuHélène Bœuf
Mar 5, 2008·Journal of Occupational Medicine and Toxicology·Gaetano CaramoriAlberto Papi
Jul 4, 2006·The Journal of Allergy and Clinical Immunology·Bernhard HomeyDonald Y M Leung
Sep 9, 2008·World Journal of Gastroenterology : WJG·Akira AndohYoshihide Fujiyama
Jan 29, 2013·World Journal of Hepatology·Lela Stefanovic, Branko Stefanovic
Jan 18, 2013·Blood·Pawel Muranski, Nicholas P Restifo
Jun 12, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jun W KimJennifer R Cochran
Apr 11, 2012·Molecular Immunology·Lindsay L JonesDario A A Vignali

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.