Cilostazol inhibits platelet-leukocyte interaction by suppression of platelet activation

Platelets
Hideki ItoT Mori

Abstract

The influence of three anti-platelet drugs, cilostazol, aspirin, and tirofiban, was investigated on platelet-leukocyte interaction by flow cytometry. When platelets and leukocytes were pre-incubated with anti-platelet drugs and stimulated by thrombin or collagen, cilostazol was found to inhibit platelet adhesion to monocytes and polymorphonuclear cells (PMNs). Similar effects were observed with anti-CD62P antibody, while aspirin and tirofiban did not appear to interfere with interaction between platelets and leukocytes. In the platelets pre-incubated with anti-platelet drugs, cilostazol significantly reduced CD62P expression and GPIIb/IIIa activation on platelet surface stimulated by thrombin or collagen. Aspirin inhibited CD62P expression and GPIIb/IIIa activation induced by collagen, but not thrombin. Tirofiban significantly blocked GPIIb/IIIa activation induced with both, and weakly inhibited CD62P expression induced by collagen. When added after stimulation of platelets, cilostazol again significantly inhibited CD62P expression and GPIIb/IIIa activation, although to a lesser extent than in the pre-incubation study. Aspirin hardly inhibited CD62P expression or GPIIb/IIIa activation, while tirofiban strongly blocked GPIIb/III...Continue Reading

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Citations

Oct 2, 2007·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Sebastián F AmerisoAntonio Ricardo La Mura
Jul 1, 2008·British Journal of Pharmacology·N E BarrettJ M Gibbins
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Jan 13, 2011·British Journal of Clinical Pharmacology·Gabriella Passacquale, Albert Ferro
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Oct 10, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hisayuki MatsunagaSoichiro Miura
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Dec 3, 2016·American Journal of Veterinary Research·Daniela HernandezTracy Stokol
Jan 9, 2021·International Immunopharmacology·Nadia Alice Vieira MottaFernanda Carla Ferreira de Brito

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