CIP2A mediates fibronectin-induced bladder cancer cell proliferation by stabilizing β-catenin

Journal of Experimental & Clinical Cancer Research : CR
Fengbin GaoZhoujun Shen

Abstract

Fibronectin (FN) is associated with tumorigenesis and progression in bladder cancer, however, the underlying mechanisms causing this remain largely unknown. Furthermore, cancerous inhibitor of protein phosphatase 2A (CIP2A) has been shown to play important regulatory roles in cancer proliferation. Here, we investigated whether FN regulates CIP2A expression to promote bladder cancer cell proliferation. The correlations of stromal FN with CIP2A and proliferating cell nuclear antigen (PCNA) expression were analyzed in a cohort bladder cancer patients. The roles of FN and CIP2A in regulating bladder cancer cell proliferation were evaluated in cell and animal models. Cycloheximide treatment was used to determine the effects of CIP2A on β-catenin stabilization. The CIP2A-β-catenin interaction was confirmed by immunofluorescence staining and co-immunoprcipitation. In this study, we found that stromal FN expression correlated positively with the levels of CIP2A and PCNA in bladder cancer tissues. Meanwhile, in human bladder cancer cell lines (T24 and J82), exogenous FN significantly promoted cell proliferation, however, CIP2A depletion inhibited this process. Furthermore, the interaction between CIP2A and β-catenin enhanced the stabili...Continue Reading

References

Dec 4, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E IoachimN Pavlidis
Feb 15, 2005·Urology·Violeta MenéndezFrancisco García-López
Aug 13, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christophe CômeJukka Westermarck
Dec 5, 2009·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Stephen PandolAnna Gukovskaya
Jul 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisabeth J M HuijbersAnna-Karin Olsson
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jun 4, 2011·Life Sciences·Nathália G AmadoJosé G Abreu
Sep 8, 2011·British Journal of Cancer·C BöckelmanA Ristimäki
Feb 22, 2012·The Journal of Cell Biology·Pengfei LuZena Werb
Aug 28, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuanyuan FangShulan Zhang
Nov 22, 2012·Cold Spring Harbor Perspectives in Biology·Jennifer L Stamos, William I Weis
Jan 24, 2013·Cancer Medicine·Lisa P HuangJason P Trama
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Oct 13, 2015·Clinical & Experimental Metastasis·Shanna A ArnoldAndries Zijlstra
Mar 31, 2016·Oncotarget·Manjola BalliuFrancesco Paoletti
Feb 24, 2017·Journal of Cancer Research and Clinical Oncology·Min TangChunli Luo

❮ Previous
Next ❯

Citations

Dec 13, 2019·Japanese Journal of Clinical Oncology·Houxin RuanChaozhao Liang
Aug 23, 2019·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Hui GaoYunbo Ma
Mar 19, 2021·Journal of Experimental & Clinical Cancer Research : CR·Sheila SpadaPaola Nisticò
Apr 10, 2021·Frontiers in Cell and Developmental Biology·Qi YuStephanie K Seidlits
Jul 10, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Zhenzhen GaoYanhong Gu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Assay
flow cytometry
xenograft
co-IP
transfection

Software Mentioned

CellQuest
SPSS
ImageJ

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.