Circ-ITGA7 sponges miR-3187-3p to upregulate ASXL1, suppressing colorectal cancer proliferation

Cancer Management and Research
Guangpu YangJinping Ma

Abstract

Background: As a class of endogenous noncoding RNAs, some circular RNAs (circRNAs) have recently been reported to play a role in the regulation of tumorigenesis and progression in colorectal cancer (CRC). However, the mechanisms by which most these circRNAs function in CRC are still unclear. Purpose: The objective of this study was to identify the role of circRNA-ITGA7 in CRC cell proliferation. Patients and methods: Human genome-wide circRNA microarray v2 analysis was used for expression profile analysis. Target genes were predicted using online bioinformatics database, including TargetScan, miRDB, miRTarbase and miRMap. Gene overexpression and silencing cell models were built using cell transfection. qRT-PCR and Western blot were performed for gene and protein expression assessment. CCK8, colony formation and cell cycle analysis were used for proliferation testing. Annexin V-FITC analysis was performed for apoptosis detection. Results: CircRNA sequencing analysis suggested that compared to that in adjacent normal control tissue, the expression of circ-ITGA7, a novel circRNA, is decreased significantly in CRC. Gain-of-function studies further demonstrated that circ-ITGA7 suppressed proliferation of CRC cells. Based on predicti...Continue Reading

Citations

Apr 29, 2020·Journal of Cellular Physiology·Xinyuan Zhao, Li Cui
Jun 10, 2020·Cell Proliferation·Hui NieChunlin Ou
May 8, 2020·International Journal of Oncology·Hao-Ying WangYong-Ning Zhou
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Mar 10, 2021·Journal of Cellular and Molecular Medicine·Anthony LiXiufen Zheng
Jun 5, 2021·European Journal of Clinical Investigation·Guifeng ZhangLi Lin
May 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardReza Vafaee
Jul 14, 2021·Journal of Experimental & Clinical Cancer Research : CR·Zhipeng JiangShuang Chen
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Methods Mentioned

BETA
transfection
RNAseq
PCR
electrophoresis
flow cytometry
RNA-seq

Software Mentioned

Modfit LT
Arraystar
SPSS
TargetScan

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