Circ09863 Regulates Unsaturated Fatty Acid Metabolism by Adsorbing miR-27a-3p in Bovine Mammary Epithelial Cells

Journal of Agricultural and Food Chemistry
Zhi ChenZhangping Yang

Abstract

Fatty acid composition plays a key role in regulating flavor and quality of milk. Therefore, in order to improve milk quality, it is particularly important to investigate regulatory mechanisms of milk fatty acid metabolism. Circular RNAs (circRNAs) regulate expression genes associated with several biological processes including fatty acid metabolism. In this study, high-throughput sequencing was used to detect differentially expressed genes in bovine mammary tissue at early lactation and peak lactation. Circ09863 profiles were influenced by the lactation stage. Functional studies in bovine mammary epithelial cells (BMECs) revealed that circ09863 promotes triglyceride (TAG) synthesis together with increased content of unsaturated fatty acids (C16:1 and C18:1). These results suggested that circ09863 is partly responsible for modulating fatty acid metabolism. Additionally, software prediction identified a miR-27a-3p binding site in the circ09863 sequence. Overexpression of miR-27a-3p in BMECs led to decreased TAG synthesis. However, overexpression of circ09863 (pcDNA-circ09863) in BMECs significantly reduced expression of miR-27a-3p and enhanced gene expression of fatty acid synthase (FASN), a target of miR-27a-3p. Overall, data s...Continue Reading

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Apr 8, 2021·Journal of Agricultural and Food Chemistry·Zhi ChenZhangping Yang

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