Circadian expression of clock genes in human oral mucosa and skin: association with specific cell-cycle phases

The American Journal of Pathology
G A BjarnasonY Ben-David

Abstract

We studied the relative RNA expression of clock genes throughout one 24-hour period in biopsies obtained from the oral mucosa and skin from eight healthy diurnally active male study participants. We found that the human clock genes hClock, hTim, hPer1, hCry1, and hBmal1 are expressed in oral mucosa and skin, with a circadian profile consistent with that found in the suprachiasmatic nuclei and the peripheral tissues of rodents. hPer1, hCry1, and hBmal1 have a rhythmic expression, peaking early in the morning, in late afternoon, and at night, respectively, whereas hClock and hTim are not rhythmic. This is the first human study to show a circadian profile of expression for all five clock genes as documented in rodents, suggesting their functional importance in man. In concurrent oral mucosa biopsies, thymidylate synthase enzyme activity, a marker for DNA synthesis, had a circadian variation with peak activity in early afternoon, coinciding with the timing of S phase in our previous study on cell-cycle timing in human oral mucosa. The major peak in hPer1 expression occurs at the same time of day as the peak in G(1) phase in oral mucosa, suggesting a possible link between the circadian clock and the mammalian cell cycle.

References

Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E ChuC J Allegra
Aug 1, 1991·Gastroenterology·K N BuchiN H Rubin
Mar 7, 1990·Journal of the National Cancer Institute·R von Roemeling, W J Hrushesky
Jan 1, 1988·Archives of Biochemistry and Biophysics·S RadparvarJ A Houghton
Nov 1, 1968·The Journal of protozoology·J J Wille, C F Ehret
Dec 1, 1983·The American Journal of Anatomy·L E SchevingL A Scheving
Jun 1, 1982·Journal of Biochemical and Biophysical Methods·R D Armstrong, R B Diasio
Jan 1, 1981·Progress in Neuro-psychopharmacology·L J GrotaG M Brown
Dec 1, 1993·Archives of Oral Biology·K A Warnakulasuriya, D G MacDonald
Apr 1, 1994·Gastroenterology·G MarraE D'Arcangelo
Jan 1, 1993·Annual Review of Physiology·C S Pittendrigh
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·T MoriC H Johnson
Apr 29, 1998·Journal of Biological Rhythms·D R Weaver
Sep 15, 1998·Biochemical and Biophysical Research Communications·S HonmaM Nomura
Oct 21, 1998·Sleep·D KatzenbergE Mignot
Feb 13, 1999·Cell·J C Dunlap
Feb 23, 1999·The American Journal of Pathology·G A BjarnasonR B Sothern
Oct 31, 2000·International Journal of Cancer. Journal International Du Cancer·D W LincolnP A Wood

❮ Previous
Next ❯

Citations

Aug 13, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Michèle TeboulGérard Milano
Feb 16, 2006·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Barbara A MurphyBarry P Fitzgerald
Jul 25, 2008·Breast Cancer Research and Treatment·Xiaoming YangWilliam J M Hrushesky
Oct 22, 2005·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Anne M CollacoMichael E Geusz
Dec 1, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·José Angel García-SáenzEduardo Díaz-Rubio
Dec 18, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zsuzsanna LengyelAndrás Dávid Nagy
Jun 7, 2003·Brain Research. Brain Research Reviews·Nicolas Cermakian, Diane B Boivin
Nov 17, 2006·Reproduction, Nutrition, Development·Lissia Pardini, Bertrand Kaeffer
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mijung YeomShin Yamazaki
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shobhan GaddameedhiAziz Sancar
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Florian SpörlAchim Kramer
Jun 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yingjuan QianXinbin Chen
Oct 20, 2009·Human Molecular Genetics·Amy S LeonardsonEric E Schadt
Jan 19, 2010·Critical Care Medicine·Beatrice HaimovichStephen F Lowry
Jul 23, 2008·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Francis LéviAlbert Goldbeter
Jan 27, 2012·Photochemistry and Photobiology·Joshua A DesotelleNihal Ahmad
Feb 22, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Althea ElliottJohn J Reiners
Nov 7, 2012·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·I V KurbatovaN N Nemova
Jan 9, 2010·Annual Review of Pharmacology and Toxicology·Georgios K PaschosGarret A FitzGerald
Jan 11, 2007·Annual Review of Pharmacology and Toxicology·Francis Levi, Ueli Schibler
Jan 9, 2010·Annual Review of Pharmacology and Toxicology·Francis LéviJean Clairambault
Dec 15, 2011·World Journal of Surgical Oncology·Yaping WangZongyou Chen
Mar 25, 2010·PLoS Computational Biology·Samuel BernardHanspeter Herzel
Jul 25, 2009·PLoS Genetics·Kevin K LinBogi Andersen
Dec 20, 2012·PloS One·Purificación Gómez-AbellánMarta Garaulet
Sep 16, 2006·Biological Chemistry·Agneta Rannug, Ellen Fritsche
Oct 13, 2011·Croatian Medical Journal·Pavel KavcicMario Poljak
Oct 1, 2012·Journal of Research in Pharmacy Practice·Pinar Erkekoglu, Terken Baydar
Oct 9, 2012·Journal of Circadian Rhythms·Lisa M WattsBarbara A Murphy
Jul 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroki R UedaSeiichi Hashimoto
Apr 9, 2014·BMC Gastroenterology·Ming-Luen HuMing-Yu Yang
Aug 12, 2014·Molecular Biology of the Cell·Tetsuya GotohCarla V Finkielstein
Feb 2, 2006·In Vitro Cellular & Developmental Biology. Animal·Bertrand Kaeffer, Lissia Pardini
May 26, 2012·Cellular and Molecular Life Sciences : CMLS·Cristina SanduMarie-Paule Felder-Schmittbuhl
Feb 6, 2008·Expert Opinion on Drug Metabolism & Toxicology·Massimo Baraldo
May 12, 2009·The Veterinary Journal·Barbara A Murphy
Dec 2, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Atilla AltinokAlbert Goldbeter
Aug 19, 2007·Advanced Drug Delivery Reviews·Atilla AltinokAlbert Goldbeter

❮ Previous
Next ❯

Related Concepts

Related Feeds