Circadian rhythmicity in murine pre-adipocyte and adipocyte cells

Chronobiology International
Daniella T OtwayJonathan D Johnston

Abstract

Adipose tissue is central to metabolic homeostasis, signaling in part through the secretion of molecules termed adipokines. Circadian rhythms play an important role in adipose physiology, with plasma adipokine concentration and approximately 20 % of the murine adipose transcriptome undergoing 24 h variation. However, due to the heterogeneity of adipose tissue and rhythmical input from both neuronal and humoral signals, the cellular basis of adipose rhythms is unclear. We tested the hypothesis that adipocyte cells contain a circadian clock that drives rhythmic mRNA expression and adipokine secretion. From the murine pre-adipocyte 3T3-L1 cell line, we generated populations of both pre-adipocytes and differentiated adipocytes. Cells were then treated with a 2 h serum pulse and sampled every 4 h over a 48 h period. Expression of clock gene, 'metabolic' gene (PPARalpha, PPARgamma, SREBP1), and adipokine mRNA was analyzed by quantitative real-time PCR, and secretion of the adipokines leptin and adiponectin was measured in culture medium from differentiated adipocytes. In pre-adipocytes, we observed robust rhythms of clock genes Per2, Rev-erbalpha, and Dbp, but not of Per1, Cry1, Bmal1, or any of the 'metabolic' genes. Adipocytes prod...Continue Reading

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Citations

Mar 29, 2014·Nutrition Research Reviews·Jonathan D Johnston
Jun 3, 2015·Chronobiology International·Edith GrosbelletEtienne Challet
Jun 15, 2011·Molecular and Cellular Endocrinology·Jonathan D Johnston
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