Circadian rhythms in the synthesis and degradation of a master clock protein KaiC in cyanobacteria.

The Journal of Biological Chemistry
Keiko ImaiHideo Iwasaki

Abstract

A circadian rhythm in the accumulation of the core clock protein KaiC has been proposed to be important for proper circadian timing in the cyanobacterium Synechococcus elongatus PCC 7942 under continuous light conditions. Cycling in the abundance of the KaiC protein is delayed to the rhythm of its mRNA by approximately 8 h, consistent with the proposed function of KaiC as a negative feedback regulator of kaiBC transcription. Here, we present temporal profiles of the synthesis and degradation of KaiC protein that determine the rhythm of its accumulation. The rate of KaiC synthesis shows a robust circadian oscillation, which is delayed to the mRNA rhythm slightly and advances the rhythm of KaiC accumulation by approximately 6 h. The stability of KaiC protein also shows circadian fluctuations, such that KaiC degradation is suppressed during the mid-subjective night. These results suggest that transcriptional, translational, and posttranslational processes are important for the proper circadian changes in KaiC accumulation. Moreover, the turnovers of the phosphorylated and non-phosphorylated forms of KaiC show robust circadian rhythms with an anti-phase relationship to each other. Interestingly, when translation was inhibited, KaiC...Continue Reading

References

Nov 18, 1994·Science·T KondoM Ishiura
Jan 1, 1993·Annual Review of Physiology·C S Pittendrigh
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T KondoC H Johnson
Feb 13, 1999·Cell·J C Dunlap
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·T NishiwakiT Kondo
Dec 2, 2000·Plant & Cell Physiology·H Iwasaki, T Kondo
Sep 5, 2001·Nature Reviews. Genetics·M W Young, S A Kay
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hideo IwasakiTakao Kondo
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stanly B WilliamsAndy C LiWang
Nov 21, 2002·The Journal of Biological Chemistry·Hakuto KageyamaHideo Iwasaki
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MoriCarl H Johnson
Mar 8, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fumio HayashiMasahiro Ishiura
Nov 18, 2003·Annual Review of Genetics·J L DittyS S Golden
Jan 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoichi NakahiraTakao Kondo
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Susan S. GoldenTakao Kondo
Sep 1, 1991·Plant Physiology·T H ChenT C Huang

❮ Previous
Next ❯

Citations

Oct 30, 2007·Nature Structural & Molecular Biology·Hiroshi ItoTakao Kondo
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·David ZwickerPieter Rein ten Wolde
May 15, 2008·Genes & Development·Yohko KitayamaTakao Kondo
Mar 20, 2010·Science·Qiong YangAlexander van Oudenaarden
Jan 1, 2008·Journal of Bacteriology·Yoriko MurayamaTakao Kondo
Nov 17, 2006·Journal of Biological Rhythms·Mark A Woelfle, Carl Hirschie Johnson
Mar 22, 2013·PLoS Computational Biology·Stefanie HertelIlka M Axmann
May 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rachelle M Smith, Stanly B Williams
Dec 28, 2007·Journal of Biotechnology·Tamaki EndohEiry Kobatake
Jan 3, 2006·Journal of Theoretical Biology·Hisako Takigawa-Imamura, Atsushi Mochizuki
Jul 25, 2017·BMC Evolutionary Biology·Nicolas M SchmellingAnika Wiegard

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.