Circadian Rhythms Influence the Severity of Sepsis in Mice via a TLR2-Dependent, Leukocyte-Intrinsic Mechanism

The Journal of Immunology : Official Journal of the American Association of Immunologists
Erica L HeipertzWendy E Walker

Abstract

Circadian rhythms coordinate an organism's activities and biological processes to the optimal time in the 24-h daylight cycle. We previously demonstrated that male C57BL/6 mice develop sepsis more rapidly when the disease is induced in the nighttime versus the daytime. In this report, we elucidate the mechanism of this diurnal difference. Sepsis was induced via cecal ligation and puncture (CLP) at zeitgeber time (ZT)-19 (2 am) or ZT-7 (2 pm). Like the males used in our prior study, female C57BL/6 mice had a worse outcome when CLP was induced at ZT-19 versus ZT-7, and these effects persisted when we pooled the data from both sexes. In contrast, mice with a mutated Period 2 (Per2) gene had a similar outcome when CLP was induced at ZT-19 versus ZT-7. Bone marrow chimeras reconstituted with C57BL/6 immune cells exhibited a worse outcome when sepsis was induced at ZT-19 versus ZT-7, whereas chimeras with Per2-mutated immune cells did not. Next, murine macrophages were subjected to serum shock to synchronize circadian rhythms and exposed to bacteria cultured from the mouse cecum at 4-h intervals for 48 h. We observed that IL-6 production oscillated with a 24-h period in C57BL/6 cells exposed to cecal bacteria. Interestingly, we obser...Continue Reading

References

Nov 20, 1998·Archives of Surgery·J SchröderF Stüber
Sep 16, 1999·Archives of Surgery·P J OffnerW L Biffl
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Niels C RiedemannPeter A Ward
Jan 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·F HALBERGJ J BITTNER
Feb 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Seung-Hee YooJoseph S Takahashi
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hongnian GuoEric L Bittman
Feb 29, 2008·American Journal of Physiology. Renal Physiology·Hideo YasudaRobert A Star
May 14, 2008·The Journal of Experimental Medicine·George PlitasRonald P DeMatteo
Oct 29, 2008·Clinics in Chest Medicine·Nicholas S WardAlfred Ayala
May 9, 2009·Nature Medicine·Richard S HotchkissThomas A Ferguson
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maren KellerBert Maier
Feb 11, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Wendy E WalkerDaniel R Goldstein
Jul 29, 2010·Physiological Reviews·Diego A Golombek, Ruth E Rosenstein
Sep 30, 2010·Journal of Biological Rhythms·Michael E HughesKarl Kornacker
Sep 25, 2012·Trends in Immunology·Alvaro ArjonaErol Fikrig
Oct 11, 2012·Journal of Leukocyte Biology·Wendy E WalkerDaniel R Goldstein
Apr 9, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meihong DengTimothy R Billiar
Aug 15, 2015·Mediators of Inflammation·Shigeto HamaguchiKazunori Tomono
Sep 16, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Evan L ChiswickDaniel G Remick
Feb 24, 2016·JAMA : the Journal of the American Medical Association·Mervyn SingerDerek C Angus
Aug 16, 2016·Biochemical and Biophysical Research Communications·Chao-Yung WangMing-Shien Wen
Aug 25, 2017·Journal of Biomedical Science·Semjon SeemannAmelie Lupp

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Citations

Dec 12, 2019·Frontiers in Immunology·Akram M ZaaqoqYoram Vodovotz
Jan 17, 2020·JCI Insight·Jeffrey A HaspelLaura A Solt
Jun 2, 2020·Frontiers in Immunology·Shan ChenJennifer J Loros
Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Claudio CostantiniMarina Maria Bellet
Oct 8, 2020·Current Protocols in Immunology·Frances V SjaastadThomas S Griffith
Nov 27, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erica L HeipertzWendy E Walker
Feb 17, 2021·Antioxidants & Redox Signaling·Fengqian ChenWei Chao
Sep 22, 2021·International Reviews of Immunology·Xu-Li FanPei-Yao Lin
Oct 4, 2020·Biochemical Pharmacology·Haritha JacobCathal J Kearney

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