Circadian tracking of nicotinamide cofactor levels in an immortalized suprachiasmatic nucleus cell line

Neuroscience
Dana D Wise, Jason B Shear

Abstract

Nicotinamide adenine dinucleotides can exhibit a daily rhythm in plants and regulate the activity of mammalian clock-like transcription factors in vitro. Because one such redox-sensitive transcription factor is present in the master circadian clock of the brain (the suprachiasmatic nuclei, SCN) and the SCN exhibits a characteristic daily rhythm in glucose usage, nicotinamide cofactors might be expected to influence, exhibit, and/or reflect biological rhythms in SCN cells. Therefore, cofactors were extracted from a model SCN cell line at 3 h intervals over 1-2 day periods and samples were analyzed by capillary electrophoresis with multiphoton excitation of fluorescence. Natively fluorescent reduced cofactors (nicotinamide adenine dinucleotide, NADH, and its phosphorylated form, NADPH) were assayed directly, and nonfluorescent oxidized cofactors (nicotinamide adenine dinucleotide, NAD, and its phosphorylated form, NADP) were enzymatically reduced to their fluorescent counterparts before analysis. In the first day after a synchronizing pulse of fetal bovine serum, a dramatic upregulation in cellular NADH content was observed, consistent with a response to serum insulin; this was accompanied by a smaller decrease in NADPH redox sta...Continue Reading

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Citations

Feb 23, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Etienne Challet
Oct 30, 2009·The European Journal of Neuroscience·Etienne ChalletPaul Pévet
Oct 11, 2014·Biochemistry·Chelsea L Gustafson, Carrie L Partch
Apr 4, 2009·Frontiers in Neuroendocrinology·Christopher M MayerDenise D Belsham
Mar 19, 2011·Journal of Neuroscience Research·Yoshiaki IsobeHitoo Nishino
Sep 23, 2006·The Journal of Biological Chemistry·Noriyuki ShiraishiMorimitsu Nishikimi

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