CircAGFG1 drives metastasis and stemness in colorectal cancer by modulating YY1/CTNNB1.

Cell Death & Disease
Lei ZhangLetian Shan

Abstract

Colorectal cancer (CRC) is a common malignancy with high occurrence and mortality worldwide. In recent years, the overall survival rate of CRC patients has been improved because of the advances in early diagnosis and therapy. However, the prognosis of CRC patients at the advanced stage is still poor due to high recurrence rate and metastasis. The function of circular RNA (circRNA) ArfGAP with FG repeats 1 (circAGFG1) has been explored in non-small-cell lung cancer and triple-negative breast cancer. Nevertheless, its role in CRC is not clear. In this study, circAGFG1 was upregulated in CRC cell lines. CircAGFG1 silencing significantly suppressed cell proliferation, migration, invasion, and stemness, while promoted cell apoptosis in CRC. Meanwhile, we found that circAGFG1 also accelerated CRC tumor growth and metastasis in vivo. Importantly, circAGFG1 activated Wnt/β-catenin pathway through regulating CTNNB1. Afterwards, YY1 was found to transcriptionally activate CTNNB1. Furthermore, circAGFG1 directly sponged miR-4262 and miR-185-5p to upregulate YY1 expression. Eventually, rescue assays demonstrated that the effect of circAGFG1 silencing on CRC cell functions was observably reversed by upregulating YY1 or CTNNB1. In brief, our...Continue Reading

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Citations

Feb 13, 2021·Cancer Management and Research·Liqiang QiSu Lu
Apr 7, 2021·Translational Neuroscience·Zihuang LiMeili Chen
May 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardReza Vafaee
May 17, 2021·Critical Reviews in Oncology/hematology·Amna MatlyJoanne Edwards
Jul 1, 2021·Oncology Letters·Mingying Zhang, Shubin Wang
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Yuqiang LiCenap Güngör
Sep 19, 2021·Cancer Cell International·Mandana Ameli-MojaradMaziar Ashrafian Bonab

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Methods Mentioned

BETA
PCR
transfection
confocal microscopy
FCS
flow cytometry
electrophoresis
xenograft
immunoprecipitation
RIP
nuclear translocation

Software Mentioned

Prism
GraphPad
SPSS
FCS Express

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