CircBPTF knockdown ameliorates high glucose-induced inflammatory injuries and oxidative stress by targeting the miR-384/LIN28B axis in human umbilical vein endothelial cells.

Molecular and Cellular Biochemistry
Wei Zhang, Yunun Sui

Abstract

Endothelial dysfunction is a primary cause of diabetes-related vascular complications, such as atherosclerosis. Accumulated research indicates that circular RNAs (circRNAs) are involved in the pathogenesis of cardiovascular disease. This study intended to explore the function and mechanism of circBPTF in high glucose (HG)-induced vascular inflammatory models. Cell model of inflammatory injury was established in human umbilical vein endothelial cells (HUVECs) with HG treatment. The expression of circBPTF, miR-384 and lin-28 homolog B (LIN28B) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability and apoptosis were assessed by cell counting kit-8 (CCK-8) and flow cytometry assay, respectively. The expression of LIN28B was also examined using western blot. The release of proinflammatory cytokines was detected by enzyme-linked immunosorbent assay (ELISA). The production of ROS, SOD and MDA was detected to assess oxidative stress. The target relationship was predicted by bioinformatics analysis and verified using dual-luciferase reporter assay and RIP assay. CircBPTF was highly regulated in HG-induced HUVECs. CircBPTF knockdown increased cell viability and suppressed cell apoptosis, the release o...Continue Reading

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Citations

Mar 13, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hengquan WanShunlin Qu
May 8, 2021·Frontiers in Medicine·Xiaoyun HeChunlin Ou
Jul 27, 2021·Oxidative Medicine and Cellular Longevity·Yanshuo HanJian Zhang
Aug 14, 2021·Frontiers in Pharmacology·Yao ZhangFukuan Du
Aug 27, 2021·Bioorganic & Medicinal Chemistry Letters·Xue LiGang Chen

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Methods Mentioned

BETA
transfection
electrophoresis
FACS
MDA
Assay
PCR
immunoprecipitation
RIP
flow cytometry
ELISA

Software Mentioned

GraphPad Prism
starBase

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