circFMN2 Sponges miR-1238 to Promote the Expression of LIM-Homeobox Gene 2 in Prostate Cancer Cells

Molecular Therapy. Nucleic Acids
Guangyi ShanQiguang Chen

Abstract

Circular RNAs (circRNAs) regulate gene expression in different malignancies. However, the molecular mechanisms that link circRNAs with the tumorigenesis of prostate cancer (PCa) are not well understood. In the present study, we attempted to provide a novel basis for targeted therapy for PCa from the aspect of circRNA-microRNA (miRNA)-mRNA interaction. We investigated the expression of circRNAs in 5 paired PCa tissues and adjacent non-tumor tissues by microarray analysis. We focused on hsa_circ_0005100, which is located on chromosome 1 and derived from FMN2, and thus we named it circFMN2. The qRT-PCR was used to detect circFMN2 and target miRNA expression in PCa tissues and cell lines. Biological functional experiments were performed to detect the effects of circFMN2 on the biological behavior of PCa cells in vivo and in vitro. Bioinformatic analysis was utilized to predict potential miRNA target sites on circFMN2. High expression of circFMN2 was associated with PCa progression. Function assays revealed that knockdown of circFMN2 significantly reduced PCa cell growth in vitro and in vivo. Finally, we found that circFMN2 acts as a competing endogenous RNA (ceRNA) for miR-1238 to regulate LIM-homeobox gene 2 (LHX2) expression. cir...Continue Reading

Citations

Jan 26, 2021·Frontiers in Cell and Developmental Biology·Wenbin ChenXiangming Mao
Nov 26, 2020·Cancer Management and Research·Haitao LiChengkuan Cai
Feb 2, 2021·International Journal of General Medicine·Xiuhua LiuYun Yang
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Jiajie FangJindan Luo
Mar 19, 2021·The International Journal of Biochemistry & Cell Biology·Chunlei ZhangDehui Chang
Aug 14, 2021·Frontiers in Cell and Developmental Biology·Fan ChaoGang Chen
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Shengxian XuHongtuan Zhang

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Methods Mentioned

BETA
Chip
flow cytometry
xenograft
immunoprecipitation
pull down
RIP
transmission electron microscopy
PCR
transfection

Software Mentioned

R
Prism
circRNA
SPSS
StarBase
Arraystar Chip
TargetScan
edgeR

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