CircGRIA1 shows an age-related increase in male macaque brain and regulates synaptic plasticity and synaptogenesis.

Nature Communications
Kaiyu XuJiali Li

Abstract

Circular RNAs (circRNAs) are abundant in mammalian brain and some show age-dependent expression patterns. Here, we report that circGRIA1, a conserved circRNA isoform derived from the genomic loci of α-mino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor subunit Gria1, shows an age-related and male-specific increase in expression in the rhesus macaque prefrontal cortex and hippocampus. We show circGRIA1 is predominantly localized to the nucleus, and find an age-related increase in its association with the promoter region of Gria1 gene, suggesting it has a regulatory role in Gria1 transcription. In vitro and in vivo manipulation of circGRIA1 negatively regulates Gria1 mRNA and protein levels. Knockdown of circGRIA1 results in an age-related improvement of synaptogenesis, and GluR1 activity-dependent synaptic plasticity in the hippocampal neurons in males. Our findings underscore the importance of circRNA regulation and offer an insight into the biology of brain aging.

References

Jan 1, 1990·Annual Review of Neuroscience·C E Finch, D G Morgan
Feb 8, 1991·Cell·J M NigroB Vogelstein
Sep 1, 1989·Neurobiology of Aging·C W CotmanD T Monaghan
Jan 1, 1989·Annals of the New York Academy of Sciences·Z S Khachaturian
Nov 1, 1987·Neurobiology of Aging·P D Coleman, D G Flood
Dec 1, 1995·General Pharmacology·P GareriG De Sarro
Mar 13, 1998·Annual Review of Psychology·B Kolb, I Q Whishaw
May 6, 1998·Current Opinion in Neurobiology·M Constantine-Paton, H T Cline
Sep 18, 2004·Trends in Neurosciences·Patrick R Hof, John H Morrison
Dec 24, 2005·Nature Reviews. Neuroscience·Sara N Burke, Carol A Barnes
May 21, 2009·Molecular Psychiatry·C S WeickertM J Webster
Jul 13, 2011·Cold Spring Harbor Perspectives in Biology·William A Catterall
Oct 28, 2011·Nature·Hyo Jung KangNenad Sestan
Dec 20, 2012·RNA·William R JeckNorman E Sharpless
Mar 1, 2013·Nature·Sebastian MemczakNikolaus Rajewsky
Mar 1, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo MostanyCarlos Portera-Cailliau
Sep 10, 2013·Cancer Research·Thomas B HansenChristian K Damgaard
Sep 17, 2013·Molecular Cell·Yang ZhangLing-Ling Chen
May 9, 2014·Nature Biotechnology·William R Jeck, Norman E Sharpless
Sep 23, 2014·RNA·Petar GlažarNikolaus Rajewsky
Sep 23, 2014·Molecular Cell·Reut Ashwal-FlussSebastian Kadener
Oct 5, 2014·Genes & Development·Dongming Liang, Jeremy E Wilusz
Feb 11, 2015·Nature Structural & Molecular Biology·Zhaoyong LiGe Shan
Apr 30, 2015·Molecular Cell·Agnieszka Rybak-WolfNikolaus Rajewsky
Apr 14, 2016·Cell Reports·Yang ZhangLing-Ling Chen
Dec 14, 2016·Scientific Reports·Hannah GrunerPedro Miura
Jan 7, 2017·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·UNKNOWN Alzheimer's Association Calcium Hypothesis Workgroup
Jan 10, 2017·Ageing Research Reviews·Mahesh Shivarama Shetty, Sreedharan Sajikumar
Mar 24, 2017·Briefings in Bioinformatics·Yuan GaoFangqing Zhao
Mar 28, 2017·Molecular Cell·Nagarjuna Reddy PamudurtiSebastian Kadener
Mar 28, 2017·Molecular Cell·Ivano LegniniIrene Bozzoni
Oct 30, 2018·Frontiers in Synaptic Neuroscience·Txomin LalanneP Jesper Sjöström
Mar 21, 2019·Cell Reports·Peifeng JiFangqing Zhao

❮ Previous
Next ❯

Citations

Feb 10, 2021·Cellular and Molecular Life Sciences : CMLS·Kaiyu XuJiali Li
May 22, 2021·Trends in Genetics : TIG·Eunah KimSeung-Jae V Lee
Jul 27, 2021·Frontiers in Molecular Neuroscience·Yue QiJun Wan

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE94027
GSE85377

Methods Mentioned

BETA
RNA-seq
PCR
circRNA-seq
FCS
electrophoresis
PCRs
Assay
transfection
in vitro transcription PCR
in vitro transcription

Software Mentioned

Image J
Stimfit
BASEscope
Metamorph
RNAscope
CIRI2
Excel
circRNA
blastn
circAtlas

Related Concepts

Related Feeds

Brain Aging

Here is the latest research on intrinsic and extrinsic factors, as well as pathways and mechanisms that underlie aging in the central nervous system.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Related Papers

American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics
Chiara MagriSergio Barlati
Clinical and Experimental Reproductive Medicine
Hossein GolestanpourMohammad Hasan Sheikhha
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Andy N Mead, David N Stephens
© 2021 Meta ULC. All rights reserved