CircHECTD1 up-regulates mucin 1 expression to accelerate hepatocellular carcinoma development by targeting microRNA-485-5p via a competing endogenous RNA mechanism.

Chinese Medical Journal
Qiao-Li JiangShuang-Zhu Wang

Abstract

Non-coding RNAs have attracted considerable attention for their vital role in cancer. The purpose of this study was to determine the effects of non-coding RNAs on hepatocellular carcinoma (HCC) and reveal their regulatory mechanism in the pathophysiological process. We measured the expression of mucin 1 (MUC1) and miR-485-5p in tissues from 15 HCC patients and in liver cancer cell lines by quantitative real-time polymerase chain reaction and Western blot, screened for aberrantly expressed microRNAs (miRNAs) by miRNA microarrays. Bioinformatics tools were used to find the miRNA and circular RNA that regulated MUC1, which were validated by RNA immunoprecipitation assay and luciferase reporter assay. Cell counting kit-8, Transwell assays, and flow cytometry were used to conduct functional experiments. Proteins were examined by western blot and immunohistochemical staining assays. Significant differences between groups were estimated using the one-way analysis of variance. A P < 0.05 was considered statistically significant. MUC1 was overexpressed in HCC tissues compared with that in paratumor tissues (normal vs. tumor, 1.007 ± 0.215 vs. 75.213 ± 18.403, t = 18.401, P < 0.001) while miR-485-5p was down-regulated (normal vs. tumor, ...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H L SangerA K Kleinschmidt
Dec 14, 2007·Nature Reviews. Cancer·Dario C Altieri
Jun 10, 2008·Biochemical and Biophysical Research Communications·Yuki OhkawaKeiko Furukawa
Apr 17, 2012·Journal of Nutrition and Metabolism·John Janez MiklavcicMichael Thomas Clandinin
Mar 1, 2013·Nature·Thomas B HansenJørgen Kjems
Mar 29, 2014·Trends in Molecular Medicine·Sritama Nath, Pinku Mukherjee
Feb 11, 2015·Nature Structural & Molecular Biology·Zhaoyong LiGe Shan
Jun 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiangjun SunYutong Wang
Sep 25, 2015·World Journal of Gastroenterology : WJG·John J MiklavcicM Tom Clandinin
Dec 4, 2015·Drug Discoveries & Therapeutics·Shaohua Ge, Dingzhi Huang
Jan 7, 2016·Frontiers in Oncology·Alejandro Parrales, Tomoo Iwakuma
Jan 24, 2016·Japanese Journal of Clinical Oncology·Yu-Xin WangJun Qian
Feb 26, 2016·Nature Reviews. Molecular Cell Biology·Ling-Ling Chen
Mar 2, 2017·The Journal of Clinical Investigation·Brian D AdamsFrank J Slack
Sep 14, 2017·Theranostics·Jie NiYong Li
Feb 7, 2018·Pharmacology & Therapeutics·Bing HanHonghong Yao
Feb 13, 2018·Journal of Hematology & Oncology·Miaoci Zhang, Yan Xin
Apr 22, 2018·Biochemical and Biophysical Research Communications·Meng WangXu-Chen Cao
May 29, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·SongTao XiangSwei Sunny Hann
Oct 12, 2018·Immunology and Cell Biology·Shuang CaoLe Zhang
Dec 15, 2018·Seminars in Cancer Biology·Esther ArnaizCharles H Lawrie
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 16, 2019·Journal of Cellular Physiology·Wei ZhaoJie Wu
Feb 24, 2019·Journal of Experimental & Clinical Cancer Research : CR·Liangliang XuMingqing Xu
May 15, 2019·Molecular Therapy. Nucleic Acids·Jie WuWei Zhao
Aug 23, 2019·Oncogene·Hasan RajabiDonald Kufe
Oct 28, 2019·Chinese Medical Journal·Dong LiXiao-Hua Zhu

❮ Previous
Next ❯

Citations

Feb 10, 2021·Journal of Cellular Physiology·Chenlong LiPeng Liang
Feb 6, 2021·Journal of Hepatocellular Carcinoma·Abdullah ElyPatrick Arbuthnot
Apr 17, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Nicolas JonckheereIsabelle Van Seuningen

❮ Previous
Next ❯

Methods Mentioned

BETA
Feature Extraction
Protein Assay
electrophoresis
PCR
immunoprecipitation
RIP
transfection
flow cytometry

Software Mentioned

GraphPad Prism
Agilent Feature Extraction
TargetScan
Gene Spring GX
Starbase

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Breast Cancer : the Journal of the Japanese Breast Cancer Society
Xiaowen WangHua Li
European Review for Medical and Pharmacological Sciences
L-L Jing, X-M Mo
Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Jiawen WuDingwei Zhang
© 2021 Meta ULC. All rights reserved