circHMGCS1-016 reshapes immune environment by sponging miR-1236-3p to regulate CD73 and GAL-8 expression in intrahepatic cholangiocarcinoma.

Journal of Experimental & Clinical Cancer Research : CR
Ya-Ping XuChuan-Yong Guo

Abstract

Accumulating evidence indicates that circRNAs may serve as essential regulators in the progression of several human cancers, but the function and mechanism of circRNAs in intrahepatic cholangiocarcinoma (ICC) are largely unknown. RNA-seq was used to assess differentially expressed circRNAs between 4 ICC and peritumor tissues. Quantitative RT-PCR and in situ hybridization were used to determine the circHMGCS1-016 expression in ICC tissues. The function and mechanism of circHMGCS1-016 were further identified via in vivo experiments. The clinical characteristics and prognostic significance of circHMGCS1-016 were analyzed by a retrospective study. The functions of circHMGCS1-016 were assessed via modifying circRNA expression in ICC cells. Moreover, the molecular mechanisms of circHMGCS1-016 in ICC cells were explored by circRNA precipitation, miRNA immunoprecipitation, SILAC and luciferase reporter assays. We identified that compared with peritumor tissues, ICC tissues expressed hsa_circ_0008621 (circHMGCS1-016) high by RNA-seq, which was further identified by qRT-PCR and in situ hybridization. Moreover, the expression of circHMGCS1-016 was revealed to be associated with survival and recurrence of ICC patients. By regulating circHM...Continue Reading

References

Jan 9, 2007·Cancer Letters·Syed Saif HasanNaheed Banu
Feb 4, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qiang GaoJia Fan
Aug 5, 2010·Cancer Research·Bin Zhang
Jul 29, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Roger L MilneDouglas F Easton
Dec 27, 2011·Cell·Ignacio Rubio-SomozaJavier Paz-Ares
Mar 1, 2013·Nature·Thomas B HansenJørgen Kjems
Apr 1, 2014·Journal of Hepatology·John BridgewaterGregory J Gores
Nov 19, 2014·Annals of Translational Medicine·Ali Hasan EbrahimMaurizio Chiriva-Internati
Apr 11, 2015·Cancer Cell·Suzanne L TopalianDrew M Pardoll
Dec 23, 2017·OncoTargets and Therapy·Rui PengDousheng Bai
Jan 30, 2018·Journal of Hepatology·Jian YuWei-Ping Zhou
Jul 31, 2018·Molecular Cell·Xiang LiLing-Ling Chen
Dec 18, 2018·Expert Opinion on Therapeutic Targets·Ghasem GhalamfarsaFarhad Jadidi-Niaragh
Feb 9, 2019·Cell·Josh N VoArul M Chinnaiyan
Mar 10, 2019·Molecular Cancer·Jing FengChunjiang He
Sep 19, 2019·Glycobiology·María V TribulattiOscar Campetella
Oct 4, 2019·Immunotherapy·Farhad Jadidi-Niaragh
Aug 11, 2020·Current Opinion in Pharmacology·Meejeon RohBin Zhang
Nov 26, 2020·Journal of Cancer Research and Clinical Oncology·Zelin HouHeguang Huang
Aug 11, 2021·Journal of Experimental & Clinical Cancer Research : CR·Yuanyuan ZhengChuanyong Guo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.