CircHMGCS1 Promotes Hepatoblastoma Cell Proliferation by Regulating the IGF Signaling Pathway and Glutaminolysis

Theranostics
Ni ZhenQiuhui Pan

Abstract

Circular RNAs (circRNAs), a novel class of endogenous RNAs, have been recently shown to participate in cellular development and several pathophysiological processes. The identification of dysregulated circRNAs and their function in cancer have attracted considerable attention. Nevertheless, the expression profile and role of circRNAs in human hepatoblastoma (HB) remain to be studied. In this report, we analyzed the expression prolife of circRNAs in HB tissues and identified circHMGCS1 (3-hydroxy-3-methylglutaryl-CoA synthase 1; hsa_circ_0072391) as a remarkably upregulated circRNA. Methods: The expression prolife of circRNAs in HB tissues were investigated through circRNA sequencing analyses. ISH and qRT-PCR assays were performed to measure the expression level of circHMGCS1. The effect of knocking down circHMGCS1 in HB cells in vitro and in vivo were evaluated by colony formation assay, flow cytometry, xenograft tumors assay and untargeted metabolomics assay. MRE analysis and dual luciferase assay were performed to explore the underlying molecular mechanisms. Results: HB patients with high circHMGCS1 expression have shorted overall survival. Knockdown of circHMGCS1 inhibits HB cells proliferation and induces apoptosis. CircHMG...Continue Reading

Citations

Apr 25, 2020·International Journal of Molecular Sciences·Yunuen Ortiz-PedrazaMarcela Lizano
Jan 14, 2021·Journal of Experimental & Clinical Cancer Research : CR·Xuehui WangLin Fang
Jan 13, 2021·Seminars in Cancer Biology·Aferin BeilerliAamir Ahmad
Jan 6, 2021·Journal of Experimental & Clinical Cancer Research : CR·Jiacheng CaoZekuan Xu
Mar 7, 2021·Biomolecules·Caterina MancarellaKatia Scotlandi
Mar 20, 2021·Journal of Cellular and Molecular Medicine·Ziwei LiTao Meng
Mar 30, 2021·Cancer Biomarkers : Section a of Disease Markers·Mujie YeKuiran Dong
Jun 13, 2021·Cancer Cell International·Jiabin YuHongting Lu
Aug 28, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Peng LiXiangdong Li

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Methods Mentioned

BETA
RNA-seq
transfection
electrophoresis
FACS
metabolomics profiling
xenograft
circRNA-seq
flow cytometry

Software Mentioned

SPSS
edgeR bioconductor package
MPEA
MEM
CircInteractome
TargetScan
XploreMET
CIRI
BWA
GraphPad Prism

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